The complete mitochondrial genome (mitogenome) of Diploprion bifasciatum was first determined. The mitogenome was 16,805 bp in length and contained 13 protein-coding genes, 24 tRNAs, 2 rRNA, and 2 non-coding regions. The overall base composition of the mitogenome was estimated to be T 25.72%, G 17.10%, A 28.24%, and C 28.94%, with a slight A + T bias of 53.96%, indicating an obvious anti-G bias commonly observed invertebrates. It shared 78.9%, 78.7%, 76.8%, and 72.5% identities with that of Grammistes sexlineatus, Epinephelus moara, Plectropomus areolatus, and Hypoplectrus gemma, respectively.
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