AI Article Synopsis
- Microalgal transformation is becoming more popular, and Agrobacterium-mediated transformation is highlighted as the most effective method for creating transgenic plants and microalgae because it's cost-effective and can transfer large DNA segments.
- In this study, researchers isolated three microalgal species from freshwater, identified them morphologically and through genetic techniques, and successfully transformed Chlorella sp., Ankistrodesmus sp., and Scenedesmus bajacalifornicus using Agrobacterium.
- The success of gene integration was confirmed through PCR and GUS assays, revealing transformation efficiencies of 12.25% for Chlorella sp., 3.5% for Ankistrodesmus sp., and
Article Abstract
Microalgal transformation has gained interest in recent years. Agrobacterium-mediated transformation remains as the most efficient method for the development of transgenic plants and microalgae due to its wide host range, inexpensive procedure and transfer of large segments of DNA. In the present study, three different microalgal species were isolated from freshwater environment and identified based on the morphological characteristics and ITS-2 region amplification. Agrobacterium-mediated transformation was successful for the isolates Chlorella sp., Ankistrodesmus sp and Scenedesmus bajacalifornicus. Gene integration and expression was confirmed by PCR amplification of hptII and GUS histochemical assay. A. tumifaciens contamination was checked by amplification of npt II gene (kanamycin resistant) which lies outside the T-border. Based on GUS assay, transformation efficiencies were found to be 12.25% for Chlorella sp. 2.96% for Scenedesmus bajacalifornicus and 3.5% for Ankistrodesmus sp.
Download full-text PDF |
Source |
|---|
Publication Analysis
Top Keywords
Similar Publications
Want AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!