AI Article Synopsis
- - The researchers developed a non-invasive platform called Pulse that uses video microscopy and image analysis to assess the function of cardiomyocytes derived from pluripotent stem cells.
- - Pulse automatically measures important cardiac parameters such as beating frequency, duration, amplitude, and variability through motion analysis of rapidly captured images.
- - The platform successfully reproduces drug effects on these stem-cell-derived cardiomyocytes and is effective for high-throughput drug screening for cardiotoxicity and studying relevant heart functions.
Article Abstract
We present a non-invasive method to characterize the function of pluripotent stem-cell-derived cardiomyocytes based on video microscopy and image analysis. The platform, called Pulse, generates automated measurements of beating frequency, beat duration, amplitude, and beat-to-beat variation based on motion analysis of phase-contrast images captured at a fast frame rate. Using Pulse, we demonstrate recapitulation of drug effects in stem-cell-derived cardiomyocytes without the use of exogenous labels and show that our platform can be used for high-throughput cardiotoxicity drug screening and studying physiologically relevant phenotypes.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4400609 | PMC |
| http://dx.doi.org/10.1016/j.stemcr.2015.02.007 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Want AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!