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| http://dx.doi.org/10.1371/journal.ppat.1004656 | DOI Listing |
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Understanding Hematopoietic Stem Cell Development through Functional Correlation of Their Proliferative Status with the Intra-aortic Cluster Architecture.
Stem Cell Reports
June 2017
Institute for Stem Cell Research, Medical Research Council Centre for Regenerative Medicine, University of Edinburgh, SCRM Bioquarter, 5 Little France Drive, Edinburgh EH16 4UU, UK. Electronic address:
During development, hematopoietic stem cells (HSCs) emerge in the aorta-gonad-mesonephros (AGM) region through a process of multi-step maturation and expansion. While proliferation of adult HSCs is implicated in the balance between self-renewal and differentiation, very little is known about the proliferation status of nascent HSCs in the AGM region. Using Fucci reporter mice that enable in vivo visualization of cell-cycle status, we detect increased proliferation during pre-HSC expansion followed by a slowing down of cycling once cells start to acquire a definitive HSC state, similar to fetal liver HSCs.
View Article and Find Full Text PDFTo be or not IIb: a multi-step process for Epstein-Barr virus latency establishment and consequences for B cell tumorigenesis.
PLoS Pathog
March 2015
Department of Molecular Genetics and Microbiology, Duke Center for Virology, Duke University Medical Center, Durham, North Carolina, United States of America.
Runx1 is required for progression of CD41+ embryonic precursors into HSCs but not prior to this.
Development
September 2014
MRC Centre for Regenerative Medicine, University of Edinburgh, Edinburgh EH16 4UU, UK
Haematopoiesis in adult animals is maintained by haematopoietic stem cells (HSCs), which self-renew and can give rise to all blood cell lineages. The AGM region is an important intra-embryonic site of HSC development and a wealth of evidence indicates that HSCs emerge from the endothelium of the embryonic dorsal aorta and extra-embryonic large arteries. This, however, is a stepwise process that occurs through sequential upregulation of CD41 and CD45 followed by emergence of fully functional definitive HSCs.
View Article and Find Full Text PDFCord blood in vitro expanded CD41 cells: identification of novel components of megakaryocytopoiesis.
J Thromb Haemost
April 2006
Department of Biochemistry, IRCCS Policlinico S. Matteo, University of Pavia, Pavia, Italy.
Background: Megakaryopoiesis represents a multi-step, often unclear, process leading to commitment, differentiation, and maturation of megakaryocytes (MKs) that release platelets.
Aim: To identify the novel genes that might help to clarify the molecular mechanisms of megakaryocytopoiesis and be regarded as potential candidates of inherited platelet defects, global gene expression of hematopoietic lineages was carried out.
Methods: Human cord blood was used to purify CD34+ stem cells and in vitro expand CD41+ cells and burst-forming unit-erythroid (BFU-E).
Multi-step fibrinogen binding to the integrin (alpha)IIb(beta)3 detected using force spectroscopy.
Biophys J
October 2005
Department of Cell and Developmental Biology, University of Pennsylvania School of Medicine, Philadelphia, PA 19104-6085, USA.
The regulated ability of integrin alphaIIbbeta3 to bind fibrinogen plays a crucial role in platelet aggregation and hemostasis. We have developed a model system based on laser tweezers, enabling us to measure specific rupture forces needed to separate single receptor-ligand complexes. First of all, we performed a thorough and statistically representative analysis of nonspecific protein-protein binding versus specific alphaIIbbeta3-fibrinogen interactions in combination with experimental evidence for single-molecule measurements.
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