AI Article Synopsis
- During adolescence, sex hormones, particularly estrogen, influence the development and regulation of cartilage cells called chondrocytes, but the specific mechanisms remain partially understood.
- The study utilized adipose-derived stem cells (ADSCs) to investigate how estrogen affects the expression of cartilage-specific genes during the chondrogenesis process, comparing control and experimental groups.
- Results showed that estrogen inhibits the expression of type II collagen and reduces aggrecan gene expression, suggesting that estrogen may have negative effects on the formation of cartilage from ADSCs.
Article Abstract
Background: During adolescence, sex hormones play an important role in regulating proliferation, differentiation, maturation, and the scheduled death of chondrocytes. Although some studies have reported the regulatory role of estrogen in the development and progression of cartilage, some of the mechanisms still remain unclear, including the role of estrogen in the expression of cartilage-specific genes in chondrogenesis process, which we cover in this study.
Materials And Methods: In the present study, we used adipose-derived stem cells (ADSCs) to differentiate into cartilage. Differentiated cartilage cells were used in the control (without estrogen E2 in the culture medium) and experimental (with estrogen in the culture medium) groups to evaluate the expression of type II collagen and aggrecan as chondrogenic genes markers, with -real-time polymerase chain reaction technique.
Results: Our results indicated that estrogen leads to inhibition of type II collagen gene expression and reduction of aggrecan gene expression.
Conclusion: Therefore, estrogen probably has negative effects on chondrogenesis process of ADSCs.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4358037 | PMC |
| http://dx.doi.org/10.4103/2277-9175.151252 | DOI Listing |
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