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Function: _error_handler
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Filename: controllers/Detail.php
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File: /var/www/html/index.php
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Function: strpos
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Function: insertAPISummary
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Due to the size of the required equipment, automated laboratory systems are often unavailable or impractical for use in small- and mid-sized laboratories. However, recent developments in automation engineering provide endless possibilities for incorporating benchtop devices. Here, the authors describe the development of a platform technology to handle sealed culture dishes. The programming is based on the Petri net method and implemented via Codesys V3.5 pbF. The authors developed a system of three independent electrical driven axes capable of handling sealed culture dishes. The device performs two difference processes. First, it automatically obtains an image of every processed culture dish. Second, a server-based image analysis algorithm provides the user with several parameters of the cultivated sample on the culture dish. For demonstration purposes, the authors developed a continuous, systematic, nondestructive, and quantitative method for monitoring the growth of a hairy root culture. New results can be displayed with respect to the previous images. This system is highly accurate, and the results can be used to simulate the growth of biological cultures. The authors believe that the innovative features of this platform can be implemented, for example, in the food industry, clinical environments, and research laboratories.
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http://dx.doi.org/10.1177/2211068215576191 | DOI Listing |
J Clin Med
November 2024
Department of Pediatric Dentistry, Asahi University School of Dentistry, Gifu 501-0296, Japan.
Dental pulp (DP) is a connective tissue composed of various cell types, including fibroblasts, neurons, adipocytes, endothelial cells, and odontoblasts. It contains a rich supply of pluripotent stem cells, making it an important resource for cell-based regenerative medicine. However, current stem cell collection methods rely heavily on the enzymatic digestion of dissected DP tissue to isolate and propagate primary cells, which often results in low recovery rates and reduced cell survival, particularly from deciduous teeth.
View Article and Find Full Text PDFJ Vis Exp
November 2024
Italian Liver Foundation;
Primary hepatocytes are a commonly used tool for in vitro liver-related studies. However, the maintenance of these cells has always been a challenge due to the rapid loss of morphology, viability, and functionality in culture. A recent approach to long-term culture is the generation of three-dimensional (3D) organoids, an in vitro tool that can recapitulate tissues in a dish based on the marvelous ability of the liver to regenerate itself.
View Article and Find Full Text PDFCureus
November 2024
Paediatrics, Dr. D. Y. Patil Medical College, Hospital and Research Centre, Dr. D.Y. Patil Vidyapeeth (Deemed to be University), Pune, IND.
The pioneering German physician and microbiologist Heinrich Hermann Robert Koch (1843-1910) made pivotal contributions to the field of bacteriology, significantly advancing the germ theory of disease. His groundbreaking research in identifying the causative agents of anthrax, tuberculosis, and cholera revolutionized medical science and public health. Koch's development of essential microbiological techniques, such as using agar for bacterial cultures and introducing the Petri dish, transformed laboratory practices.
View Article and Find Full Text PDFNat Commun
December 2024
Department of Chemical and Biomolecular Engineering, Korea Advanced Institute of Science and Technology (KAIST), 291 Daehak-ro, Yuseong-gu, Daejeon, Republic of Korea.
Urogynecology (Phila)
December 2024
Department of Surgery, University of Arizona College of Medicine, Tucson, AZ.
Importance: Vaginal fibroblast function is altered in people with pelvic organ prolapse. Thus, it is important to study vaginal fibroblasts to better understand the pathophysiology of prolapse.
Objective: This study aimed to compare 3 culturing methods of primary vaginal fibroblasts.
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