Background: Metabolites are underappreciated for their effect on coagulation. Taurocholic acid (TUCA), a bile acid, has been shown to regulate cellular activity and promote fibrin sealant degradation. We hypothesize that TUCA impairs whole blood clot formation and promotes fibrinolysis.
Methods: TUCA was exogenously added to whole blood obtained from volunteers. A titration from 250 μM-750 μM was used due to biologic relevance. Whole blood mixtures were assayed using thrombelastography for clot strength (maximum amplitude [MA]) and fibrinolysis (LY30) quantification. Tranexamic acid was used to block plasmin-mediated fibrinolysis. Platelet microfluidics were performed. A proteomic analysis was completed on citrated plasma obtained from a shock and resuscitation rat model.
Results: Fibrinolysis increased when 750-μM TUCA was added to whole blood (median LY30 0.08-5.7, P = 0.010) and clot strength decreased (median MA of 53.3-43.8, P = 0.010). The addition of tranexamic acid, to a 750-μM TUCA titration, partially reversed the induced fibrinolysis (LY30: without 7.7 versus with 2.7) and the decrease in clot strength (MA: without 48.2 versus with 53.2), but did not reverse the effects to whole blood levels. Platelet function reduced by 50% in the presence of 100-μM TUCA. Rats had a median 52-fold increase in TUCA, after a shock state that stayed elevated after resuscitation.
Conclusions: TUCA reduces clot strength and promotes fibrinolysis. The clot strength reduction is attributable to platelet inhibition. This metabolic effect on coagulation warrants further investigation, as localized areas of the body, with high levels of bile acid, may be at risk for postoperative bleeding.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4838575 | PMC |
| http://dx.doi.org/10.1016/j.jss.2015.01.046 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
The Correlation of Hemostatic Parameters with the Development of Early Sepsis-Associated Encephalopathy. A Retrospective Observational Study.
J Crit Care Med (Targu Mures)
October 2024
Carol Davila University of Medicine and Pharmacy, Bucharest, Romania.
Introduction: Sepsis-associated encephalopathy (SAE) is one of the most common complications seen both in early and late stages of sepsis, with a wide spectrum of clinical manifestations ranging from mild neurological dysfunction to delirium and coma. The pathophysiology of SAE is still not completely understood, and the diagnosis can be challenging especially in early stages of sepsis and in patients with subtle symptoms.
Aim Of The Study: The objective of this study was to assess the coagulation profile in patients with early SAE and to compare the hemostatic parameters between septic patients with and without SAE in the first 24 hours from sepsis diagnosis.
Evaluating platelet concentrates by platelet indices, thromboelastography, and flow cytometry.
Asian J Transfus Sci
May 2023
Department of Hematology, All India Institute of Medical Sciences, New Delhi, India.
Introduction: Platelet transfusion has been therapeutically used in patients with thrombocytopenia and platelet function defects over the years. The use of advanced techniques may add value in assessing the quality of platelet products. The aim of the study was to assess stored platelet concentrates (PCs) prepared in blood banks for platelet indices, clot strength, and platelet function.
View Article and Find Full Text PDFFish scale gelatin/diatom biosilica composite hemostasis sponge with ultrafast dispersing and in situ gelation for hemorrhage control.
Int J Biol Macromol
January 2025
College of Marine Life Science, Ocean University of China, 5# Yushan Road, Qingdao 266003, Shandong Province, China; Sanya Oceanographic Institute, Ocean University of China, Floor 7, Building 1, Yonyou Industrial Park, Yazhou Bay Science & Technology City, Sanya, Hainan Province, China. Electronic address:
Rapid control of hemorrhage is vital in first-aid and surgery. As representative of emergency hemostatic materials, inorganic porous materials achieve rapid hemostasis through concentrating protein coagulation factors by water adsorption to accelerate the coagulation reaction process, however their efficacy is often limited by the insufficient contact of material with blood and the lack of blood clot strength. Herein, we report an ultrafast dispersing and in situ gelation sponge (SG/DB) based on anchoring interface effect for hemorrhage control using freeze drying method after mixing fish scale gel (SG) and tert-butyl alcohol (TBA) pre-crystallized diatom biosilica (DB).
View Article and Find Full Text PDFThromboelastography may assess the effect of anticoagulation reversal in intracranial hemorrhage.
J Stroke Cerebrovasc Dis
January 2025
Department of Neurology, University of Iowa College of Medicine, United States; Department of Epidemiology, University of Iowa College of Public Health, United States; Department of Neurosurgery, University of Iowa College of Medicine, United States.
Article Synopsis
- Intracranial hemorrhage (ICH) is a serious risk for patients on oral anticoagulants, and there's a need for biomarkers to evaluate the effectiveness of reversal agents like activated prothrombin complex concentrate (aPCC).
- A study was conducted in an emergency department with adult patients experiencing factor Xa inhibitor-related ICH to assess changes in thromboelastography (TEG) following aPCC reversal.
- Results showed a significant decrease in TEG R-time shortly after aPCC administration, but it returned to baseline levels by 12 and 24 hours, suggesting TEG R-time could be a valuable biomarker to monitor anticoagulation effects in these patients.
Haemotoxicity of snakes: a review of pathogenesis, clinical manifestations, novel diagnostics and challenges in management.
Trans R Soc Trop Med Hyg
January 2025
Department of Clinical Medicine, Faculty of Medicine, University of Colombo, P.O. 00800, Sri Lanka.
Haemotoxicity is the most common complication of systemic envenoming following snakebite, leading to diverse clinical syndromes ranging from haemorrhagic to prothrombotic manifestations. Key haematological abnormalities include platelet dysfunction, venom-induced consumption coagulopathy, anticoagulant coagulopathy and organ-threatening thrombotic microangiopathy. Diagnostic methods include the bedside whole blood clotting test, laboratory coagulation screening and other advanced methods such as thromboelastogram and clot strength analysis.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!