Background: During the last decade, the prevalence of foodborne diseases due to contaminated food as well as the outbreaks of diseases due to Shiga toxin-producing Escherichia coli (STEC) strains has increased.
Objectives: The aim of this study was to evaluate the prevalence and antibiotic resistance pattern of STEC strains in lettuce samples. Since lettuce is used as a raw vegetable in salads, the rates of infections caused by this vegetable are high.
Materials And Methods: A total of 100 samples collected from Tehran, Iran, were transported to the laboratory, homogenized by a stomacher in E. coli broth containing cefixime, and cultured on MacConkey agar medium. Their DNA was extracted by boiling method and polymerase chain reaction (PCR) was performed, using five primers targeting the stx1, stx2, fliCh7, rbfO157, and eaeA genes. Susceptibility testing against ampicillin, imipenem, cephalosporin, tetracycline, aminoglycosides, chloramphenicol and quinolones was performed using disk diffusion method.
Results: Eight samples were positive for presence of STEC strains, three contained stx1, five contained stx2, and one sample was positive for presence of both rbfO157 and fliCh7. They were susceptible to all the antibiotics except for ampicillin and tetracycline.
Conclusions: This study indicated the contamination of lettuce by STEC strains and its possible role as the source of infection. Resistance to both tetracycline and ampicillin may be considered as an emergency alarm for a multidrug resistance of STEC strains.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4332234 | PMC |
| http://dx.doi.org/10.5812/jjm.12346 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Hybrid strains of enterotoxigenic/Shiga toxin-producing , United Kingdom, 2014-2023.
J Med Microbiol
January 2025
NIHR Health Protection Research Unit in Gastrointestinal Infections, University of Liverpool, Liverpool, UK.
Diarrhoeagenic (DEC) pathotypes are defined by genes located on mobile genetic elements, and more than one definitive pathogenicity gene may be present in the same strain. In August 2022, UK Health Security Agency (UKHSA) surveillance systems detected an outbreak of hybrid Shiga toxin-producing /enterotoxigenic (STEC-ETEC) serotype O101:H33 harbouring both Shiga toxin () and heat-stable toxin (). These hybrid strains of DEC are a public health concern, as they are often associated with enhanced pathogenicity.
View Article and Find Full Text PDFAn outbreak of Shiga toxin-producing serotype O103:H2 associated with unpasteurized soft cheese, England and Wales, 2022.
Epidemiol Infect
January 2025
Gastrointestinal Infections and Food Safety (One Health) Division, Clinical and Public Health Group, UK Health Security Agency, London, UK.
In July 2022, a genetically linked and geographically dispersed cluster of 12 cases of Shiga toxin-producing (STEC) O103:H2 was detected by the UK Health Security Agency using whole genome sequencing. Review of food history questionnaires identified cheese (particularly an unpasteurized brie-style cheese) and mixed salad leaves as potential vehicles. A case-control study was conducted to investigate exposure to these products.
View Article and Find Full Text PDFAn efficient, high-throughput enrichment system for the rapid detection of E. coli at low concentrations in water.
Anal Chim Acta
February 2025
School of Ophthalmology & Optometry, Eye Hospital, Wenzhou Medical University, Wenzhou, 325027, China; Changchun Institute of Optics, Fine Mechanics and Physics, Chinese Academy of Sciences, Changchun, 130033, China; University of Chinese Academy of Sciences, Beijing, 100049, China; State Key Laboratory of Applied Optics, Changchun, 130033, China; Key Laboratory of Advanced Manufacturing for Optical Systems, Chinese Academy of Sciences, Changchun, 130033, China. Electronic address:
Certain virulent strains of Escherichia coli (E. coli), notably the enterohemorrhagic serotype O157:H7, are recognized for causing diarrhea, gastroenteritis, and a range of illnesses that pose significant risks to public health and the safety of drinking water supplies. Early detection and management of E.
View Article and Find Full Text PDFPrevalence and Zoonotic Risk of Multidrug-Resistant in Bovine Subclinical Mastitis Milk: Insights Into the Virulence and Antimicrobial Resistance.
Food Sci Nutr
January 2025
Department of Dairy Science, Faculty of Veterinary, Animal and Biomedical Sciences Sylhet Agricultural University Sylhet Bangladesh.
The emergence of antibiotic-resistant microorganisms has made antimicrobial resistance a global issue, and milk is a potential source for the propagation of resistant bacteria causing zoonotic diseases. Subclinical mastitis (SCM) cases, often overlooked and mixed with normal milk in dairy farms, frequently involve , which can spread through contaminated milk. We conducted this study to determine the prevalence of virulence genes, antibiotic resistance genes (ARGs), antimicrobial susceptibility, and the genetic relatedness of multidrug-resistant (MDR) Shiga toxin-producing (STEC) isolated from SCM milk.
View Article and Find Full Text PDFPhytochemicals Controlling Enterohemorrhagic (EHEC) Virulence-Current Knowledge of Their Mechanisms of Action.
Int J Mol Sci
January 2025
Department of Bacterial Molecular Genetics, Faculty of Biology, University of Gdansk, Wita Stwosza 59, 80-308 Gdansk, Poland.
Enterohemorrhagic (EHEC) is a common pathotype of that causes numerous outbreaks of foodborne illnesses. EHEC is a zoonotic pathogen that is transmitted from animals to humans. Ruminants, particularly cattle, are considered important reservoirs for virulent EHEC strains.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!