[Research on structural and functional characteristics (indicators) of apoptosis of human blood lymphocytes under the influence of carbon monoxide and ultraviolet (UV)-radiation].

The changes in the level of expression of membrane CD95 receptor, the structural state of DNA, parameters of biochemiluminescence of human blood lymphocytes under conditions of exposure to carbon monoxide (60-90 min) and UV-radiation (240-390 nm) at the doses of 151, 453 and 755 J/m2 have been investigated. The decrease in the level of expression of Fas-markers on the surface of immunocytes after incubation in the atmosphere at (60-90 min), the absence of changes in the DNA structure and the decreased intensity of lipid peroxidation processes in the cells were also found. It is established that UV-light (151-755 J/m2) exhibits a proapoptotic action, as evidenced by the increase in the expression of CD95 receptors on the surface of human blood lymphocytes and the decreased electrophoretic mobility of DNA in UV-irradiated cells. However, the process of a programmed cell death is not leaking ("apoptotic ladder" on electrophoregramme in the conditions of the experiment is missing). It is shown that the combined effects of carbon monoxide and UV-light in the cells of the human blood leads to a decrease in the sensitivity of the membrane CD95 receptors to the action of UV-radiation. It is revealed that carbon monoxide can contribute to blocking the processes of peroxide oxidation of lipids (POL) and, as a consequence, activate antioxidant properties of cells. It was concluded that the CO molecule in physiological concentrations is likely to have the antiapoptotic effect in relation to lymphocytes.