AI Article Synopsis
- In fission yeast, microtubules help center the nucleus by pushing against the cell's edge, but the specific role of kinesin-8 motors in this process was previously unclear.
- Researchers created a physical model showing that kinesin-8 motors promote microtubule catastrophe, which aids in nuclear positioning.
- Experimental results supported the model's predictions, demonstrating that with kinesin-8 motors, the nucleus centers better and predicting how quickly a displaced nucleus can return to the center.
Article Abstract
In fission yeast, microtubules push against the cell edge, thereby positioning the nucleus in the cell center. Kinesin-8 motors regulate microtubule catastrophe; however, their role in nuclear positioning is not known. Here we develop a physical model that describes how kinesin-8 motors affect nuclear centering by promoting a microtubule catastrophe. Our model predicts the improved centering of the nucleus in the presence of motors, which we confirmed experimentally in living cells. The model also predicts a characteristic time for the recentering of a displaced nucleus, which is supported by our experiments where we displaced the nucleus using optical tweezers.
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| http://dx.doi.org/10.1103/PhysRevLett.114.078103 | DOI Listing |
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