Transgene silencing is often associated with multicopy integrations, which occur frequently during plant transformation. Transgene expression can be restored in a number of multicopy loci by converting them to single copy. This chapter describes a plant transformation protocol based on use of the Cre-lox system, which allows conversion of a multicopy transgene locus into single copy. The strategy is based on designing a transformation vector with lox sites, developing transgenic lines, and introducing Cre activity to initiate Cre-lox recombination, which leads to the simplification of a multicopy locus to a single- or low-copy state. This method is compatible with both gene gun and Agrobacterium-mediated gene delivery and should be particularly useful for crops that are difficult to transform.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1007/978-1-4939-2453-0_6 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
The ABC transporter A7 modulates neuroinflammation via NLRP3 inflammasome in Alzheimer's disease mice.
Alzheimers Res Ther
January 2025
Translational Neurodegeneration Research and Neuropathology Lab, Department of Clinical Medicine (KlinMed), Medical Faculty, University of Oslo (UiO) and Section of Neuropathology Research, Department of Pathology (PAT), Clinics for Laboratory Medicine (KLM), Oslo University Hospital (OUS), Sognsvannsveien 20, Oslo, NO-0372, Norway.
Background: Specific genetic variants in the ATP-binding cassette transporter A7 locus (ABCA7) are associated with an increased risk of Alzheimer's disease (AD). ABCA7 transports lipids from/across cell membranes, regulates Aβ peptide processing and clearance, and modulates microglial and T-cell functions to maintain immune homeostasis in the brain. During AD pathogenesis, neuroinflammation is one of the key mechanisms involved.
View Article and Find Full Text PDFDiverse Cre recombinase expression pattern in Albumin-Cre driver rats.
Exp Anim
January 2025
Division of Animal Genetics, Laboratory Animal Research Center, Institute of Medical Science, The University of Tokyo.
Rats (Rattus norvegicus) have been widely utilized as model animals due to their physiological characteristics, making them suitable for surgical and long-term studies. They have played a crucial role in biomedical research, complementing studies conducted in mice. The advent of genome editing technologies has facilitated the generation of genetically modified rat strains, advancing studies in experimental animals.
View Article and Find Full Text PDFDifferentially spliced mitochondrial CYP419A1 contributes to ethiprole resistance in Nilaparvata lugens.
Insect Biochem Mol Biol
January 2025
Centre for Ecology and Conservation, Biosciences, University of Exeter, Penryn Campus, Penryn, Cornwall, UK. Electronic address:
The brown planthopper Nilaparvata lugens is one of the most economically important pests of cultivated rice in Southeast Asia. Extensive use of insecticide treatments, such as imidacloprid, fipronil and ethiprole, has resulted in the emergence of multiple resistant strains of N. lugens.
View Article and Find Full Text PDFA versatile site-directed gene trap strategy to manipulate gene activity and control gene expression in Caenorhabditis elegans.
PLoS Genet
January 2025
Department of Integrative Biology, University of Wisconsin-Madison, Madison, Wisconsin, United States of America.
The ability to manipulate gene activity and control transgene expression is essential to study gene function. While several genetic tools for modifying genes or controlling expression separately are available for Caenorhabditis elegans, there are no genetic approaches to generate mutations that simultaneously disrupt gene function and provide genetic access to the cells expressing the disrupted gene. To achieve this, we developed a versatile gene trap strategy based on cGAL, a GAL4-UAS bipartite expression system for C.
View Article and Find Full Text PDFFLOWERING LOCUS C-like mediates low-ambient-temperature-induced late flowering in chrysanthemum.
J Exp Bot
January 2025
State Key Laboratory of Crop Genetics and Germplasm Enhancement and Utilization, Key Laboratory of Landscaping, Ministry of Agriculture and Rural Affairs, Key Laboratory of Biology of Ornamental Plants in East China, National Forestry and Grassland Administration, College of Horticulture, Nanjing Agricultural University, Nanjing 210095, China.
The flowering time of Chrysanthemum morifolium predominantly depends on day length but is also sensitive to ambient temperature. However, the mechanisms underlying the response of chrysanthemum to ambient temperature are mainly unknown. This study identified a MADS-box transcription factor called CmFLC-like, a representative low ambient temperature-responsive factor induced in chrysanthemum leaves and shoot apical meristems at 15°C.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!