AI Article Synopsis
- The study focused on β-Cyclodextrin (β-CD)-grafted dextrans to see how different spacer arm lengths affect the stability and activity of the enzyme invertase.
- The researchers tested these modified dextrans alone and in combination with trehalose in freeze-dried formulations to analyze enzyme activity after freeze-drying and heat treatment.
- Results showed that spacer arm length significantly influenced polymer-enzyme interactions and enzyme stability, evidenced by changes in glass transition temperature (Tg) related to the chemical modifications made to the dextran backbone.
Article Abstract
β-Cyclodextrin (β-CD)-grafted dextrans with spacer arms of different length were employed to evaluate the impact of supramolecular interactions on invertase activity. The modified dextrans were used as single additives or combined with trehalose in freeze-dried formulations containing invertase. Enzyme activity conservation was analyzed after freeze-drying and thermal treatment. The change of glass transition temperature (Tg ) was also evaluated and related to effective interactions. Outstanding differences on enzyme stability were mainly related to the effect of the spacer arm length on polymer-enzyme interactions, since both the degree of substitution and the molecular weight were similar for the two polymers. This change of effective interactions was also manifested in the pronounced reduction of Tg values, and were related to the chemical modification of the backbone during oxidation, and to the attachment of the β-CD units with spacer arms of different length on dextran.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1002/btpr.2067 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Micellar casein and sodium caseinate supramolecular structure differently impacts subsequent food intake in pigs.
Food Res Int
January 2025
Institut Numecan, INSERM, INRAE, Univ Rennes, Rennes, France. Electronic address:
Dietary protein reduces energy intake in following meals by signaling directly or indirectly to the brain. We recently observed differences in plasma amino acid kinetics and intra-gastric behavior between micellar casein (MC) and sodium caseinate (SC) in pigs, two factors that impact food intake. Our objective was to clarify whether the supramolecular structure of casein, given as a preload to pigs, impacts on subsequent food intake.
View Article and Find Full Text PDFSupramolecular transparent plastic engineering covalent-and-supramolecular polymerization.
Mater Horiz
January 2025
College of Chemistry and Chemical Engineering, Hunan University, Hunan 410082, P. R. China.
Supramolecular glass and plastic are a new generation of artificial transparent materials that exhibit excellent optical behavior and processability. However, owing to inherent deficiencies in their mechanical toughness and long-term stability, supramolecular materials lack the potential for functionalization and application. Inspired by the toughening phenomena in biological systems, a synergistic covalent-and-supramolecular polymerization strategy was applied to construct plastic-like supramolecular materials with high transmittance the solvent-free one-pot amidation of thioctic acid and (poly)amines.
View Article and Find Full Text PDFDendrimers, Dendrons, and the Dendritic State: Reflection on the Last Decade with Expected New Roles in Pharma, Medicine, and the Life Sciences.
Pharmaceutics
November 2024
The National Dendrimer & Nanotechnology Center, NanoSynthons LLC, Mt. Pleasant, MI 48858, USA.
This perspective begins with an overview of the major impact that the dendron, dendrimer, and dendritic state (DDDS) discovery has made on traditional polymer science. The entire DDDS technology is underpinned by an unprecedented new polymerization strategy referred to as step-growth, amplification-controlled polymerization (SGACP). This new SGACP paradigm allows for routine polymerization of common monomers and organic materials into precise monodispersed, dendritic macromolecules (i.
View Article and Find Full Text PDFNMR spectroscopy derived plasma biomarkers of inflammation in human populations: Influences of age, sex and adiposity.
PLoS One
January 2025
Australian National Phenome Center and Center for Computational and Systems Medicine, Health Futures Institute, Murdoch University, Perth, Western Australia, Australia.
Understanding the distribution and variation in inflammatory markers is crucial for advancing our knowledge of inflammatory processes and evaluating their clinical utility in diagnosing and monitoring acute and chronic disease. 1H NMR spectroscopy of blood plasma and serum was applied to measure a composite panel of inflammatory markers based on acute phase glycoprotein signals (GlycA and GlycB) and sub-regions of the lipoprotein derived Supramolecular Phospholipid Composite signals (SPC1, SPC2 and SPC3) to establish normal ranges in two healthy, predominantly white cohorts from Australia (n = 398) and Spain (n = 80; ages 20-70 years). GlycA, GlycB, SPC1 and SPC3 were not significantly impacted by age or sex, but SPC2 (an HDL-related biomarker) was significantly higher in women across all age ranges by an average of 33.
View Article and Find Full Text PDFLaser-induced graphene functionalized cationic porphyrin: fabrication, characterization, and intra-supramolecular electron transfer process.
RSC Adv
January 2025
Nanoscience Program, Institute of Basic and Applied Sciences, Egypt-Japan University of Science and Technology New Borg El-Arab City Alexandria Egypt
We report herein a facile synthesis, characterization, and the electron transfer reaction of a novel light-harvesting material composed of laser-induced graphene (LIG) functionalized with the photoactive 5,10,15,20-tetrakis(4-trimethylammoniophenyl)porphyrin tetra(-toluenesulfonate) dye (TTMAPP). LIG was easily fabricated on the surface of a polyimide sheet using VersaLASER 3.6 (VLS 3.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!