AI Article Synopsis
- - Coronaviruses (CoVs) are assembled in the membrane compartments of cells and exit in vesicles, but the specific role of the envelope (E) protein during this process is not completely understood.
- - The study focused on the localization and movement of the E protein in mouse hepatitis CoV A59 (MHV), finding that it resides in the ERGIC and Golgi, with its ends positioned differently within the cell.
- - Techniques like fluorescence recovery after photobleaching (FRAP) indicated that the E protein moves within the ERGIC/Golgi membranes, supporting its important role in the assembly of CoVs at these locations.
Article Abstract
Coronaviruses (CoVs) assemble at endoplasmic reticulum Golgi intermediate compartment (ERGIC) membranes and egress from cells in cargo vesicles. Only a few molecules of the envelope (E) protein are assembled into virions. The role of E in morphogenesis is not fully understood. The cellular localization and dynamics of mouse hepatitis CoV A59 (MHV) E protein were investigated to further understanding of its role during infection. E protein localized in the ERGIC and Golgi with the amino and carboxy termini in the lumen and cytoplasm, respectively. E protein does not traffic to the cell surface. MHV was genetically engineered with a tetracysteine tag at the carboxy end of E. Fluorescence recovery after photobleaching (FRAP) showed that E is mobile in ERGIC/Golgi membranes. Correlative light electron microscopy (CLEM) confirmed the presence of E in Golgi cisternae. The results provide strong support that E proteins carry out their function(s) at the site of budding/assembly.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4550588 | PMC |
| http://dx.doi.org/10.1016/j.virol.2015.02.005 | DOI Listing |
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