Several organisms irrespective of their complexity in structure and function have an inbuilt circadian rhythm. Zebrafish could be used as an alternate model animal in sleep research as it exhibits similar sleep-wake dynamics as mammals and Drosophila. In this study, we have analysed the adult zebrafish brain for its differential proteome and gene expression during perturbed light/dark cycle. A total of 53 and 25 proteins including sncb, peroxiredoxins and TCR alpha were identified based on two-dimensional gel electrophoresis Fourier transform mass spectrometer/ion trap tandem mass spectrometer and differential in-gel electrophoresis MALDI TOF MS/MS analysis, respectively, with at least 1.5-fold changes between the control and experimental brains. Real time-polymerase chain reaction revealed that many circadian pathway-associated genes, such as per1b, bmal1b, cry1b, bmal2 and nr1d2, were differentially regulated during continuous light/dark exposures. It is hypothesized that the differential regulation of these genes might lead to the discovery of potential diagnostic markers for gaining insight into the light/dark-associated stress in humans.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1111/jsr.12287 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Genome-wide identification, characterization and expression pattern analysis of HAK/KUP/KT potassium transporter gene family in potato.
Front Plant Sci
January 2025
College of Agronomy, Inner Mongolia Agricultural University, Hohhot, Inner Mongolia, China.
The HAK/KUP/KT (High-affinity K transporters/K uptake permeases/K transporters) is the largest and most dominant potassium transporter family in plants, playing a crucial role in various biological processes. However, our understanding of HAK/KUP/KT gene family in potato ( L.) remains limited and unclear.
View Article and Find Full Text PDFGenome-wide identification of the wall-associated kinase gene family and their expression patterns under various abiotic stresses in soybean ( (L.) Merr).
Front Plant Sci
January 2025
College of Agriculture and Biology, Liaocheng University, Liaocheng, China.
The wall-associated kinase (WAK) gene family encodes functional cell wall-related proteins. These genes are widely presented in plants and serve as the receptors of plant cell membranes, which perceive the external environment changes and activate signaling pathways to participate in plant growth, development, defense, and stress response. However, the WAK gene family and the encoded proteins in soybean (Glycine max (L.
View Article and Find Full Text PDFIdentification of and genes and their expression profiling under biotic and abiotic stress in pigeon pea ( [L.] Millspaugh).
Front Plant Sci
January 2025
National Institute of Plant Biotechnology, Indian Council of Agricultural Research (ICAR), New Delhi, India.
The methylation- demethylation dynamics of RNA plays major roles in different biological functions, including stress responses, in plants. mA methylation in RNA is orchestrated by a coordinated function of methyl transferases (writers) and demethylases (Erasers). Genome-wide analysis of genes involved in methylation and demethylation was performed in pigeon pea.
View Article and Find Full Text PDFImplication of fibroblast growth factor 7 in ovarian cancer metastases and patient survival.
Front Oncol
January 2025
Gynecologic Oncology Section, Stephenson Cancer Center, Obstetrics and Gynecology Department, University of Oklahoma Health Sciences Center, Oklahoma City, OK, United States.
Background/objectives: Patients with ovarian cancer commonly experience metastases and recurrences, which contribute to high mortality. Our objective was to better understand ovarian cancer metastasis and identify candidate biomarkers and drug targets for predicting and preventing ovarian cancer recurrence.
Methods: Transcripts of 770 cancer-associated genes were compared in cells collected from ascitic fluid versus resected tumors of an ES-2 orthotopic ovarian cancer mouse model.
A Prognostic Riskscore Model Related to Infection in Stomach Adenocarcinoma.
Int J Genomics
January 2025
Department of General Medicine, Chongqing University Central Hospital, Chongqing Emergency Medical Center, Chongqing Key Laboratory of Emergency Medicine, Chongqing, China.
() is associated with the development of various stomach diseases, one of the major risk factors for stomach adenocarcinoma (STAD). The infection score between tumor and normal groups was compared by single-sample gene set enrichment analysis (ssGSEA). The key modules related to infection were identified by weighted gene coexpression network analysis (WGCNA), and functional enrichment analysis was conducted on these module genes.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!