AI Article Synopsis
- A straightforward fluorometric technique is introduced for detecting single-nucleotide alterations in RNA using graphene oxide and an RNA-cleaving DNAzyme.
- The method involves annealing a fluorescent DNA probe (F-DNA) to RNA fragments created by the DNAzyme that targets mutant RNA.
- The formation of the F-DNA-RNA duplex reduces the quenching effect on F-DNA fluorescence caused by graphene oxide, enhancing detection.
Article Abstract
We report a simple fluorometric method for detection of single-nucleotide changes in RNA using graphene oxide (GO) and RNA-cleaving DNAzyme. The fluorescent DNA probe (F-DNA) was annealed to RNA fragments generated by RNA cleavage with DNAzyme specific to mutant RNA. The F-DNA-RNA duplex attenuated the quenching of F-DNA fluorescence by GO.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1039/c4cc09603g | DOI Listing |
Publication Analysis
Top Keywords
detection single-nucleotide
8
single-nucleotide changes
8
changes rna
8
rna graphene
8
graphene oxide
8
rna
5
fluorescence-based detection
4
oxide dnazyme
4
dnazyme report
4
report simple
4
Similar Publications
Want AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!