AI Article Synopsis
- The study investigates how different types of stem cells from rats (ASCs and BMSCs) behave on a titanium dioxide (TiO2) layer used in bone implants.
- The researchers examined the growth patterns, proliferation rates, and the ability of these cells to differentiate into bone-forming cells by looking for specific markers associated with osteogenesis.
- Results indicated that the TiO2 layer positively affected ASCs' growth and differentiation, while also suggesting it might enhance BMSCs' osteodifferentiation, highlighting the importance of using both cell types in evaluating biomaterials for regenerative medicine.
Article Abstract
The biocompatibility of the bone implants is a crucial factor determining the successful tissue regeneration. The aim of this work was to compare cellular behavior and osteogenic properties of rat adipose-derived multipotent stromal cells (ASCs) and bone marrow multipotent stromal cells (BMSCs) cultured on metallic substrate covered with TiO2 sol-gel-derived nanolayer. The morphology, proliferation rate, and osteogenic differentiation potential of both ASCs and BMSCs propagated on the biomaterials were examined. The potential for osteogenic differentiation of ASCs and BMSCs was determined based on the presence of specific markers of osteogenesis, that is, alkaline phosphatase (ALP), osteopontin (OPN), and osteocalcin (OCL). Additionally, the concentration of calcium and phosphorus in extracellular matrix was determined using energy-dispersive X-ray spectroscopy (SEM-EDX). Obtained results showed that TiO2 layer influenced proliferation activity of ASCs, which manifested by shortening of population doubling time and increase of OPN secretion. However, characteristic features of cells morphology and growth pattern of cultures prompted us to conclude that ultrathin TiO2 layer might also enhance osteodifferentiation of BMSCs. Therefore in our opinion, both populations of MSCs should be used for biological evaluation of biomaterials compatibility, such results may enhance the area of investigations related to regenerative medicine.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4331160 | PMC |
| http://dx.doi.org/10.1155/2015/651097 | DOI Listing |
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