AI Article Synopsis
- The study explores how the Four-jointed kinase influences the distribution of cadherins Fat and Dachsous in the Drosophila wing, focusing on long-range patterning gradients.
- Using computational modeling and in vivo assays like fluorescence recovery after photobleaching, the researchers tested various scenarios to understand Four-jointed's role in cadherin binding.
- The findings reveal that Four-jointed promotes Fat's binding to Dachsous while inhibiting Dachsous's binding to Fat, with a more significant impact on Fat, explaining how opposing phosphorylation gradients shape their distribution and polarity in the wing.
Article Abstract
To understand how long-range patterning gradients are interpreted at the cellular level, we investigate how a gradient of expression of the Four-jointed kinase specifies planar polarised distributions of the cadherins Fat and Dachsous in the Drosophila wing. We use computational modelling to test different scenarios for how Four-jointed might act and test the model predictions by employing fluorescence recovery after photobleaching as an in vivo assay to measure the influence of Four-jointed on Fat-Dachsous binding. We demonstrate that in vivo, Four-jointed acts both on Fat to promote its binding to Dachsous and on Dachsous to inhibit its binding to Fat, with a bias towards a stronger effect on Fat. Overall, we show that opposing gradients of Fat and Dachsous phosphorylation are sufficient to explain the observed pattern of Fat-Dachsous binding and planar polarisation across the wing, and thus demonstrate the mechanism by which a long-range gradient is interpreted.
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Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4338440 | PMC |
| http://dx.doi.org/10.7554/eLife.05789 | DOI Listing |
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