AI Article Synopsis
- - Samples of low-molecular weight chitosan were created through enzymatic hydrolysis with a specific fungal enzyme, achieving molecular weights from 8 to 24 kDa and similar deacetylation levels.
- - The optimal hydrolysis conditions were determined to be a pH of 5.6, a temperature of 37°C, a 1/800 enzyme/substrate ratio, and a reaction time of 15 to 60 minutes.
- - This research aims to provide chitosan samples with varying characteristics, allowing for effective comparisons in subsequent laboratory and live organism experiments.
Article Abstract
Samples of low-molecular weight chitosan with molecular masses of 8-24 kDa, identical deacetylation degrees (85%), and polydispersity indexes soluble at pH 5-7 were obtained by enzymatic hydrolysis using an enzyme complex from the micelial fungi Myceliophthora fergusii with yields of 50-80%. The optimal conditions for hydrolysis were found (pH 5.6, 37 degrees C, an enzyme/substrate ratio 1/800, 15-60 min). The obtainment of chitosan sample sets with different characteristics will enable the selection of the most efficient ones for comparison in in vitro/in vivo experiments.
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