Background: Rhizoma Chuanxiong (RC) is the dried rhizome of Ligusticum chuanxiong Hort., and various types of processed Rhizoma Chuanxiong (PRC) are widely used in China. However, quality assurance and quality control of these processed medicines remain challenging. This study aims to investigate the chemical compositions of various PRC preparations by a high-performance liquid chromatography (HPLC) coupled with diode array detection (DAD) method.
Methods: A HPLC-DAD method with validation was developed for PRC samples. Seven batches of plant samples from two processing methods, stir-frying and steaming, were analyzed by the HPLC-DAD method. Common peaks in PRC chromatograms were chosen to calculate their relative retention time (RRT) and relative peak area (RPA), and similarity analyses of the chromatographic fingerprints were conducted by Similarity Evaluation System for Chromatographic Fingerprint of Traditional Chinese Medicine software (Version 2004 A).
Results: In the 24-h stability test, the relative standard deviation for the RRT and RPA was less than 0.07% and 2.57%, respectively. The precision was less than 0.08% for the RRT and 2.48% for the RPA. The repeatability for the RRT and RPA was less than 0.03% and 2.64%, respectively. The similarities between the seven PRC batches were range from 0.956 to 0.990. After stir-frying or steaming, the amount of ferulic acid in PRC was much higher than that in the raw material.
Conclusions: The fingerprint analysis of PRC by different processing methods was feasible by HPLC-DAD.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4336472 | PMC |
| http://dx.doi.org/10.1186/s13020-015-0031-3 | DOI Listing |
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Article Synopsis
- Chuanxiong Rhizoma (CX), derived from the Ligusticum chuanxiong plant, is recognized in traditional Chinese medicine for its healing properties related to blood circulation and pain relief, and has a history documented in the Chinese Pharmacopoeia from 1963 to 2020.
- This study aims to thoroughly review CX's botany, traditional applications, chemical compounds, pharmacological effects, toxicity, quality control, and its absorption and metabolism in the body.
- CX has over 400 identified compounds and is used in treating various health issues, especially those affecting the cardiovascular, liver, kidney, and nervous systems, exhibiting numerous pharmacological benefits like anti-inflammatory and antioxidant effects.
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