AI Article Synopsis
- TCDD (2,3,7,8-Tetrachlorodibenzo-p-dioxin) is a highly toxic compound linked to reproductive issues, including decreased fertility and developmental defects.
- TCDD was found to significantly reduce estradiol-17β (E2) production in human luteinizing granulosa cells when tested at various low concentrations during an in-vitro fertilization study.
- The reduction in E2 secretion correlates with lower mRNA levels of key enzymes in the estrogen biosynthesis pathway, indicating a time-dependent effect rather than a dose-dependent one.
Article Abstract
2,3,7,8-Tetrachlorodibenzo-p-dioxin (TCDD) is the most toxic congener of the polyhalogenated aromatic hydrocarbons (PAH), which causes anatomical abnormalities and developmental defects, impairs ovulation and reduces fertility. TCDD's endocrine-disrupting effects are, in part, caused by a direct action at the ovary. Herein we investigated the in-vitro effects of environmentally relevant doses of TCDD on estradiol-17β (E2) production by human luteinizing granulosa cells (hLGC) obtained from women stimulated for in-vitro fertilization (IVF). TCDD at all concentrations tested (3.1fM, 3.1pM and 3.1nM) significantly decreased E2 secretion when assayed for by radioimmunoassay (RIA). Herein we confirm that TCDD alters E2 secretion by hLGC in a time-, not dose-dependent fashion and are the first to show decreases in E2 secretion with fM concentrations of TCDD. Using real-time quantitative PCR (RT-qPCR), the decreased E2 secretion correlates with a decrease in the mRNA expression levels two enzymes in the estrogen biosynthesis pathway: CYP11A1 and CYP19A1.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4426074 | PMC |
| http://dx.doi.org/10.1016/j.reprotox.2015.02.003 | DOI Listing |
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