The crude methanol extracts and fractions of the root and stem barks of Dillenia serrata Thunb. showed 64% to 73% inhibition on the production of prostaglandin E2 (PGE2) in lipopolysaccharide-induced human whole blood using a radioimmunoassay technique. Three triterpenoids isolated from the root bark of the plant, koetjapic (1), 3-oxoolean-12-en-30-oic (2), and betulinic (3) acids, exhibited significant concentration-dependent inhibitory effects on PGE2 production with IC50 values of 1.05, 1.54, and 2.59 μM, respectively, as compared with the positive control, indomethacin (IC50 = 0.45 μM). Quantification of compounds 1 and 3 in the methanol extracts and fractions were carried out by using a validated reversed-phase high performance liquid chromatography (RP-HPLC) method. The ethyl acetate fraction of the stem bark showed the highest content of both compound 1 (15.1%) and compound 3 (52.8%). The strong inhibition of the extracts and fractions on cyclooxygenase-2 (COX-2) enzymatic activity was due to the presence of their major constituents, especially koetjapic and betulinic acids.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6272585 | PMC |
| http://dx.doi.org/10.3390/molecules20023206 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
In silico-based investigation of the molecular mechanism of Artocarpus communis seed hexane fraction against metabolic syndrome.
J Mol Model
January 2025
Department of Biochemistry, Faculty of Basic Medical Science, Olabisi Onabanjo University, Sagamu Campus, Ago Iwoye, Ogun State, Nigeria.
Context: The medications for metabolic syndromes are very minimal and the available are not effective and show adverse effects. There is a huge need for the development of effective and safe drugs to battle metabolic syndromes. In this context, our study aimed to decipher the key molecules from Artocarpus communis seed hexane fraction and their possible mechanism of action against metabolic syndrome.
View Article and Find Full Text PDFDifferential DNA Extraction from Lateral Flow Immunochromatographic Tests via the EZ1 Advanced XL System.
Methods Protoc
January 2025
The Center for Forensic Science Research and Education, 206 Welsh Road, Horsham, PA 19440, USA.
This differential extraction protocol details the steps for isolating DNA from sample pads used in lateral flow immunochromatographic (LFI) tests, particularly for cases involving mixed biological samples such as semen and menstrual blood, or other evidence related to sexual assault. This procedure utilizes a differential extraction technique applied to sample pads from immunochromatographic tests, where the sample pads serve as the substrate. The method involves two sequential lysis steps to effectively separate non-sperm and sperm fractions, enabling the targeted isolation of distinct cell types for downstream DNA analysis.
View Article and Find Full Text PDFGreenhouse Gas Emissions and Decarbonization Potential of Global Fired Clay Brick Production.
Environ Sci Technol
January 2025
Laboratory of Construction Materials, École Polytechnique Fédérale de Lausanne, CH-1015 Lausanne, Switzerland.
Fired clay bricks (FCBs) are a dominant building material globally due to their low cost and simplicity of production, especially in low- and middle-income countries. With a projected rising housing demand, commensurate growth in brick demand is anticipated, the production of which could result in significant greenhouse gas (GHG) emissions. Robust models are needed to estimate brick demand and emissions to systematically address decarbonization pathways.
View Article and Find Full Text PDFNumerical study on the prediction of oil recovery rates in unconventional reservoirs at high temperatures using ecologically friendly hybrid nanofluids.
Heliyon
January 2025
School of Sciences, Xi'an Technological University, Xi'an, 710021, China.
Although oil extraction is indispensable for meeting worldwide energy demands and ensuring industrial sustainability, various hazards are observed. Therefore, this study examined the chemical oil recovery-related environmental consequences concerning water, soil, ecosystem, and human health damages. A numerical analysis explored the mathematical model for oil extraction from unconventional sources by utilising 3D porous prism geometries under high-temperature conditions.
View Article and Find Full Text PDF[Simultaneous determination of 51 indazole-type synthetic cannabinoids in urine and blood by online solid-phase extraction-liquid chromatography-linear ion trap mass spectrometry].
Se Pu
February 2025
School of Chemistry and Chemical Engineering, Guangxi University, Nanning 530004, China.
To evade legal controls, new psychoactive substances (NPS), which have been designed as substitutes for traditional and synthetic drugs, are gradually dominating the drug market. Synthetic cannabinoids (SCs), which account for the majority of NPS, are rapidly being derivatized; consequently, controlling increasing abuse by merely listing individual compounds is difficult. Therefore, China has included the entire SC category of SCs listed as legal controlled substances since July 1, 2021.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!