Identification of five Shiga toxin-producing Escherichia coli genes by Luminex microbead-based suspension array.

J Microbiol Methods

U.S. Food & Drug Administration, Center for Food Safety and Applied Nutrition, Office of Regulatory Science, College Park, MD 20740, USA. Electronic address:

Published: April 2015

To rapidly identify the presence of potentially virulent O157:H7 and non-O157 Shiga toxin-producing Escherichia coli (STEC), a PCR-based Luminex suspension assay was developed to detect the genes coding for four virulence factors (stx1, stx2, eae, and ehxA) plus the O157:H7-specific +93 uidA single nucleotide polymorphism.

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http://dx.doi.org/10.1016/j.mimet.2015.02.008DOI Listing

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