AI Article Synopsis
- ProEx C is a new assay that targets specific proteins to improve urine cytology detection of urothelial carcinoma (UC), showing promise over the existing UroVysion assay.
- In a study involving 27 urine specimens, ProEx C exhibited higher sensitivity (88.9%) and specificity (77.8%) compared to UroVysion, which had sensitivity and specificity of 55.6% and 44.4%, respectively.
- The findings suggest that ProEx C is a more effective diagnostic tool for identifying UC in urine specimens, with statistically significant results (P = 0.0033) indicating its superiority over UroVysion.
Article Abstract
Background: Detection of urothelial carcinoma (UC) by urine cytology can be challenging. Recently, ProEx C has been studied as a marker to improve detection of UC. ProEx C is an assay targeting expression of topoisomerase IIa and minichromosome maintenance protein-2 and is currently utilized to assist in diagnoses of the gynecological specimens. In this study, we compared the utility of ProEx C and UroVysion in urine specimens.
Materials And Methods: Twenty-seven urine specimens with UroVysion assay analysis and surgical biopsy follow-up were selected. The smears were stained with ProEx C. ProEx C and UroVysion assay results were separated into two categories based on surgical biopsy follow-up (benign or neoplastic). Surgical biopsy diagnoses were used as the gold standard for comparative evaluation of the two assays. The surgical follow-up was 9 benign, 2 low grade, and 16 high grade UCs.
Results: The sensitivity was 88.9% for ProEx C and 55.6% for UroVysion, while the specificity was 77.8% for ProEx C and 44.4% for UroVysion. Positive predictive value was 88.9% for ProEx C and 66.7% for UroVysion. Negative predictive value was 77.8% and 33.3% for ProEx C and UroVysion, respectively. Using the two-tailed paired t-test, P value of 0.033 was obtained when ProEx C stain was compared with the UroVysion assay.
Conclusion: ProEx C immunocytochemistry has a more favorable performance than fluorescent in-situ hybridization with a significant difference between the two assays using paired two-tail t-test (P = 0.0033).
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4325383 | PMC |
| http://dx.doi.org/10.4103/1742-6413.149845 | DOI Listing |
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