AI Article Synopsis
- The study investigates the varying expression of the GOF-18ΔPE-GFP Pou5f1 (Oct3/4) transgene in mouse primordial germ cell (PGC) populations within the aorta-gonad-mesonephros (AGM) region.
- Researchers identified distinct populations of OCT3/4 positive cells that co-express various PGC and hematopoietic markers, indicating a link between germ cells and blood precursor cells.
- Findings reveal that certain Oct3/4-GFP positive cells exhibit strong in vitro hematopoietic colony-forming abilities, suggesting that these cells may have dual roles in germline and blood cell development.
Article Abstract
In the present paper, starting from the observation of heterogeneous expression of the GOF-18ΔPE-GFP Pou5f1 (Oct3/4) transgene in putative mouse PGC populations settled in the aorta-gonad-mesonephros (AGM) region, we identified various OCT3/4 positive populations showing distinct expression of PGC markers (BLIMP-1, AP, TG-1, STELLA) and co-expressing several proteins (CD-34, CD-41, FLK-1) and genes (Brachyury, Hox-B4, Scl/Tal-1 and Gata-2) of hematopoietic precursors. Moreover, we found that Oct3/4-GFP(weak) CD-34(weak/high) cells possess robust hematopoietic colony forming activity (CFU) in vitro. These data indicate that the cell population usually considered PGCs moving toward the gonadal ridges encompasses a subset of cells co-expressing several germ cell and hematopoietic markers and possessing hematopoietic activity. These results are discussed within of the current model of germline segregation.
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| http://dx.doi.org/10.1016/j.mod.2015.02.002 | DOI Listing |
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