A public health response relies upon rapid and reliable confirmation of disease by diagnostic assays. Here, we detail the design and validation of two variola virus-specific real-time PCR assays, since previous assays cross-reacted with newly identified cowpox viruses. The assay specificity must continually be reassessed as other closely related viruses are identified.
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|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4365196 | PMC |
| http://dx.doi.org/10.1128/JCM.03613-14 | DOI Listing |
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