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Effect of drinking water salinity on lactating cows' water and feed intake, milk yield, and rumen physiology.
Animal
December 2024
Department of Ruminant Science, Institute of Animal Science, Agricultural Research Organization, Rishon Lezion 7528809, Israel. Electronic address:
Use of desalinated seawater in arid and semiarid regions for domestic, industrial, and agricultural purposes is on the rise. Consequently, in those regions, drinking water offered to lactating cows has lower salinity and mineral concentrations than in the past. Although water with total dissolved solids (TDSs) of up to 1 000 ppm is considered safe for drinking, lower salinity level may affect rumen physiology, feed and water intake, or milk yield.
View Article and Find Full Text PDFFair charging management of PHEVs in radial distribution networks with DG resources-a case study.
Sci Rep
December 2024
Department of Electrical Engineering, Gachsaran Branch, Islamic Azad University, Gachsaran, Iran.
Considering the widespread use of PHEVs in advanced societies and the issues ahead, researchers' thinking has focused more on this issue. The important issue is that the use of EVs is increasing due to the advantages, but the necessary infrastructure for their charging stations in the distribution networks does not exist. The high penetration level of EVs can create a potential risk for the existing distribution network; the fair charging of EVs has a special value.
View Article and Find Full Text PDFMAYER-ROKITANSKY-KUSTER-HAUSER SYNDROME. LAPAROSCOPIC SIGMOID VAGINOPLASTY FOR THE TREATMENT OF VAGINAL AGENESIS - SINGLE CENTER EXPERIENCE IN GEORGIA. CASE REPORT.
Georgian Med News
September 2024
2Clinic Caraps Medline, Tbilisi, Georgia.
Introduction And Hypothesis: Sigmoid vaginoplasty is a safe and acceptable procedure for vaginal agenesis with good cosmetic results and acceptable complications rate. Sigmoid colon vaginoplasty is the treatment of choice because of its large lumen, thick walls resistant to trauma, adequate secretion allowing lubrication, not necessitating prolonged dilatation, and short recovery time. We investigate the feasibility, safety, and clinical therapeutic effect of laparoscopic sigmoid vaginoplasty in women with Mayer-Rokitansky-Kuster-Hauser (MRKH) syndrome.
View Article and Find Full Text PDFCRISPR-Cas9 Mediated Gene Deletion in Human Pluripotent Stem Cells Cultured Under Feeder-Free Conditions.
J Vis Exp
November 2024
Department of Biochemistry and Molecular Biology, College of Medicine and Health Sciences, United Arab Emirates University; Zayed Center for Health Sciences, United Arab Emirates University; ASPIRE Precision Medicine Research Institute Abu Dhabi (PMRI-AD), United Arab Emirates University;
The CRISPR-Cas9 system for genome editing has revolutionized gene function studies in mammalian cells, including stem cells. However, the practical application of this technique, particularly in pluripotent stem cells, presents certain challenges, such as being time- and labor-intensive and having low editing efficiency. Here, we describe the generation of a CRISPR-mediated gene knockout in a human embryonic stem cell (hESC) line stably expressing sgRNAs for the L2HGDH gene, using a highly efficient and stable lentiviral-mediated gene delivery system.
View Article and Find Full Text PDFLaboratory maintenance and care of Rhodnius prolixus (Hemiptera: Reduviidae) and other Triatominae.
J Med Entomol
November 2024
The Centers for Disease Control and Prevention, Division of Parasitic Diseases and Malaria, Entomology Branch, Atlanta, GA 30329, USA.
Triatomines (Hemiptera: Reduviidae) are hematophagous insects that transmit Trypanosoma cruzi, the etiological agent for Chagas disease, to humans and other mammals. As medically important vectors, species such as Rhodnius prolixus (Hemiptera: Reduviidae) have long been used as a model organism for physiological studies. Laboratory rearing of triatomines is needed to support vector and parasite research.
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