Reversed-phase high-performance liquid chromatography (RP-HPLC) with online absorbance and fluorescence detections was used for the evaluation of Suwannee River natural organic matter (SRNOM) and its fractions A, B, and C+D, obtained by conventional size exclusion chromatography-polyacrylamide gel electrophoresis (SEC-PAGE) setup, for which the electrophoretic mobility (EM) and the absorptivity varied in the order C+D > B > A, and the molecular size (MS) in the opposite order. Analysis of SRNOM and its fractions in part of their relative irreversible adsorption on C18-column and relative distribution of eluted from the column matter on hydrophobic and hydrophilic peaks showed that hydrophobicity of fractions decreased in order: A > B > C+D. The online fluorescence detection showed that SRNOM and its fractions contained at least three groups of humic substances (HS)-like fluorophores with emission maxima at 435, 455-465, and 455/420 nm and two protein-like fluorophores with emission maxima at around 300 and 340 nm. The HS-like fluorophore with emission maximum at 435 nm was located in the hydrophilic peak in all the samples. Those with maxima at 455-465 nm were detected in hydrophobic peaks of fractions A and B. SEC-PAGE setup followed by RP-HPLC allowed us to develop new approach of SRNOM separation on less heterogeneous compounds mixture for their further study and structural identification.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1007/s11356-015-4188-1 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Resolving Uncertainties in the Quantification of Trace Elements within Organic-Rich Boreal Rivers for AF4-UV-ICP-MS Analysis.
Anal Chem
May 2024
Department of Renewable Resources, University of Alberta, Edmonton AB T6G 2H1, Canada.
Article Synopsis
- Over the past few decades, asymmetric flow field-flow fractionation (AF4) has gained recognition for effectively separating colloid-associated trace elements in water samples, but there are still unknowns regarding measurement artifacts at low concentrations.
- A study using boreal river water revealed that significant trace element losses (up to 48%) occur due to adsorption onto system surfaces, which may also lead to cross-contamination.
- To address these issues, preconditioning the AF4 system with natural organic matter is proposed to reduce contamination and improve measurement accuracy in DOM-rich waters.
Characterization and quantification of silver complexes with dissolved organic matter by size exclusion chromatography coupled to ICP-MS.
J Hazard Mater
March 2024
Key Laboratory of Water and Sediment Sciences of Ministry of Education, School of Environment, Beijing Normal University, Beijing 100875, China; State Key Laboratory of Water Environment Simulation, School of Environment, Beijing Normal University, Beijing 100875, China. Electronic address:
The fate and behavior of silver in aquatic systems is intricately determined by its interactions with dissolved organic matter (DOM). In this study, we have introduced a method for identification and quantification of silver-DOM complexes using size exclusion chromatography-inductively coupled plasma mass spectrometry (SEC-ICP-MS). Our findings revealed that silver(I) was weakly bound to Suwannee River humic acid, fulvic acid, and natural organic matter (SRHA, SRFA, and SRNOM) in various media, resulting in facile dissociation during chromatographic separation.
View Article and Find Full Text PDFArsenic-dissolved organic matter complexation in water soluble extracts from lignite.
Chemosphere
November 2023
Department of Geosciences, Auburn University, USA.
Arsenic in groundwater is a global threat to public health. Recently, As mobility has been tied to the concentration and chemical characteristics of dissolved organic matter (DOM) through formation of As-DOM complexes. To date, there has been a wide range of DOM types studied to understand As-DOM interactions, but most of these have focused on surface water derived materials and not groundwater DOM.
View Article and Find Full Text PDFNew Insights into the Role of Natural Organic Matter in Fe-Cr Coprecipitation: Importance of Molecular Selectivity.
Environ Sci Technol
September 2023
Guangdong Provincial Key Laboratory of Environmental Pollution Control and Remediation Technology, School of Environmental Science and Engineering, Sun Yat-sen University, Guangzhou 510275, P. R. China.
Coprecipitation of Fe/Cr hydroxides with natural organic matter (NOM) is an important pathway for Cr immobilization. However, the role of NOM in coprecipitation is still controversial due to its molecular heterogeneity and diversity. This study focused on the molecular selectivity of NOM toward Fe/Cr coprecipitates to uncover the fate of Cr via Fourier transform-ion cyclotron resonance-mass spectrometry (FT-ICR-MS).
View Article and Find Full Text PDFThe characteristics of CDOM structural composition and the effect on indirect photodegradation of sulfamerazine.
Sci Total Environ
August 2023
Key Laboratory of Sustainable Development of Marine Fisheries, Ministry of Agriculture and Rural Affairs, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071, China; National Key Laboratory of Mariculture Biobreeding and Sustainable Goods, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071, China.
Sulfamerazine (SM) is a commonly used antibiotic and have been widely used to control various bacterial infectious diseases. The structural composition of colored dissolved organic matter (CDOM) is known to be a major factor that influences the indirect photodegradation of SM, yet the influence mechanism remains unknown. In order to understand this mechanism, CDOM from different sources was fractionated using ultrafiltration and XAD resin, and characterized using UV-vis absorption and fluorescence spectroscopy.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!