AI Article Synopsis
- The report details the creation and analysis of new three-chain insulin analogs with altered structural stability through chemical synthesis and enzymatic processing.
- The investigation focused on the 'insulin fold', a crucial structural feature of insulin, to assess its importance.
- Findings revealed that these analogs exhibited significantly reduced activity at insulin receptors compared to standard insulin, highlighting the essential role of the 'insulin fold' in insulin's biological function.
Article Abstract
This report describes the chemical synthesis and biological characterization of novel three-chain insulin analogs with a destabilized secondary structure. The analogs, obtained by chemical synthesis via a single-chain precursor and selective enzymatic digestion, were used to investigate the role of the highly conserved 'insulin fold'. Biological characterization through in vitro biochemical signaling showed extremely low activity at each insulin receptor when compared with native insulin. We conclude that the 'insulin fold' is a structural foundation that supports insulin biological action.
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| http://dx.doi.org/10.1002/psc.2744 | DOI Listing |
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