AI Article Synopsis
- NIRF is a new E3 ubiquitin ligase that interacts with HBc, leading to its degradation, which impacts HBV replication.
- The study demonstrates that NIRF significantly inhibits HBV DNA replication and the secretion of viral proteins (HBsAg and HBeAg) in both human liver cells and mouse models.
- NIRF also decreases the acetylation of H3 histones associated with HBV cccDNA, indicating a role in the regulation of HBV's replication cycle.
Article Abstract
Np95/ICBP90-like RING finger protein (NIRF), a novel E3 ubiquitin ligase, has been shown to interact with HBc and promote its degradation. This study investigated the effects of NIRF on replication of hepatitis B virus (HBV) and the mechanisms. We have shown that NIRF inhibits replication of HBV DNA and secretion of HBsAg and HBeAg in HepG2 cells transfected with pAAV-HBV1.3. NIRF also inhibits the replication and secretion of HBV in a mouse model that expressed HBV. NIRF reduces acetylation of HBV cccDNA-bound H3 histones. These results showed that NIRF is involved in the HBV replication cycle not only through direct interaction with HBc but also reduces acetylation of HBV cccDNA-bound H3 histones.
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| http://dx.doi.org/10.1089/dna.2014.2714 | DOI Listing |
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