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Revealing NOD1-Activating Gram-Positive Gut Microbiota via in Vivo Labeling with a meso-Diaminopimelic Acid Probe.
ACS Chem Biol
January 2025
Institute of Molecular Medicine, Shanghai Key Laboratory for Nucleic Acid Chemistry and Nanomedicine, Renji Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200127, China.
As an important receptor in a host's immune and metabolic systems, NOD1 is usually activated by Gram-negative bacteria having -diaminopimelic acid (-DAP) in their peptidoglycan (PGN). But some atypical Gram-positive bacteria also contain -DAP in their PGN, giving them the potential to activate NOD1. The prevalence of -DAP-type Gram-positive bacteria in the gut, however, remains largely unknown.
View Article and Find Full Text PDFTracking Somatic Mutations for Lineage Reconstruction.
Methods Mol Biol
January 2025
Department of Computer Science and Applied Mathematics, Weizmann Institute of Science, Rehovot, Israel.
The human genome is composed of distinct genomic regions that are susceptible to various types of somatic mutations. Among these, Short Tandem Repeats (STRs) stand out as the most mutable genetic elements. STRs are short repetitive polymorphic sequences, predominantly situated within noncoding sectors of the genome.
View Article and Find Full Text PDFThree new terpenes with potential antibacterial and anti-COVID-19 activities from the stems of R. Br.
Nat Prod Res
January 2025
School of Pharmaceutical Science, Guangzhou University of Chinese Medicine, Guangzhou, P. R. China.
The extract of the stems of R. Br. yielded three new terpenes () including two diterpenes and one triterpene, named euryachins C-E, as well as three known diterpenes ().
View Article and Find Full Text PDFA cell-free gene expression system for prototyping and gene expression analysis.
Appl Environ Microbiol
December 2024
Department of Microbiology, Harvard Medical School, Boston, Massachusetts, USA.
is an obligate anaerobic, Gram-positive bacterium that produces toxins. Despite technological progress, conducting gene expression analysis of under different conditions continues to be labor-intensive. Therefore, there is a demand for simplified tools to investigate the transcriptional and translational regulation of .
View Article and Find Full Text PDFCharacteristics of viral ovarian tumor domain protease from two emerging orthonairoviruses and identification of Yezo virus human infections in northeastern China as early as 2012.
J Virol
December 2024
State Key Laboratory of Pathogen and Biosecurity, Academy of Military Medical Sciences, Beijing, China.
Emerging tick-borne orthonairovirus infections pose a growing global concern, with limited understanding of the viral ovarian tumor-like cysteine proteases (vOTUs) encoded by novel orthonairoviruses. These vOTUs, a group of deubiquinylases (DUBs), disrupt the innate immune response. Yezo virus (YEZV), a recently discovered pathogenic orthonairovirus, was first reported in Japan in 2021.
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