AI Article Synopsis
- Autoinducer-2 (AI-2) is a communication molecule used by bacteria to coordinate behavior through a process called Quorum Sensing, which is important for regulating infections in humans and foodborne pathogens.
- The study presents a new biosensing system that utilizes a method called Fluorescence Resonance Energy Transfer (FRET) to detect AI-2 very quickly and with high sensitivity.
- This biosensing system can identify AI-2 at extremely low concentrations and has been successfully tested on clinical samples, including saliva and blood serum.
Article Abstract
Autoinducer-2 (AI-2) is a Quorum Sensing (QS) molecule utilized by bacteria in interspecies communication. More recently, it is identified to be vital in regulating QS pathways in a number of human and foodborne pathogens. Methods to detect AI-2 in a rapid and highly sensitive manner can help in the early detection of bacterial infections. Herein, we describe a rapid, selective, and highly sensitive protein based biosensing system employing the Fluorescence Resonance Energy Transfer (FRET) between a protein fusion LuxP-EGFP and 7-diethylamino-3-[N-(2-maleimidoethyl)carbamoyl]coumarin (MDCC). The developed biosensing system, which can detect AI-2 at subnanomolar levels, was successfully applied to detect AI-2 in clinical samples such as saliva and blood serum.
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| http://dx.doi.org/10.1021/ac504172f | DOI Listing |
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