We explored the use of matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) for identification of Fusobacterium nucleatum subspecies. MALDI-TOF MS spectra of five F. nucleatum subspecies (animalis, fusiforme, nucleatum, polymorphum, and vincentii) were analyzed and divided into four distinct clusters, including subsp. animalis, nucleatum, polymorphum, and fusiforme/vincentii. MALDI-TOF MS with the modified SARAMIS database further correctly identified 28 of 34 F. nucleatum clinical isolates to the subspecies level.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4365237 | PMC |
| http://dx.doi.org/10.1128/JCM.00239-15 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Optimizing Red Light-Based Photodynamic Therapy for Effective Bactericidal Action Against Subspecies.
Pathogens
November 2024
Department of Microbiology, Osaka Dental University, Hirakata 573-1121, Japan.
(), a key pathogen implicated in periodontal disease, contributes to oral biofilm maturation and is linked to development of systemic diseases like colorectal cancer and liver cirrhosis. Photodynamic therapy (PDT) combined with 5-aminolevulinic acid (5-ALA) treatment (ALA-PDT) selectively targets by inducing porphyrin accumulation. The bactericidal effect of red light-based PDT on has not been evaluated previously.
View Article and Find Full Text PDFelicits subspecies-specific responses in human neutrophils.
Front Cell Infect Microbiol
October 2024
Periodontal Research Group, Birmingham School of Dentistry, Institute of Clinical Sciences, The University of Birmingham, Birmingham, United Kingdom.
as a Gram-negative anaerobe plays a key bridging role in oral biofilms. It is involved in periodontal and extraoral diseases, the most prominent being colorectal cancer. Five subspecies are recognised: and .
View Article and Find Full Text PDFReexamining the role of subspecies in clinical and experimental studies.
Gut Microbes
October 2024
Division of Biomaterial and Biomedical Sciences, School of Dentistry, Oregon Health & Science University (OHSU), Portland, OR, USA.
Article Synopsis
- - The Gram-negative anaerobic species began as a harmless part of the human mouth but is now seen as a significant pathogen linked to various oral and non-oral diseases.
- - It was historically divided into four subspecies, thought to be equally harmful, but recent research shows varying pathogenic potentials among these subspecies.
- - This review explores the history of these subspecies and highlights recent findings to improve understanding for future research in clinical and lab settings.
Comparative genomics reveal a novel phylotaxonomic order in the genus Fusobacterium.
Commun Biol
September 2024
Scientific Institute IRCCS E. MEDEA, Bioinformatics, Bosisio Parini, Italy.
Fusobacteria have been associated to different diseases, including colorectal cancer (CRC), but knowledge of which taxonomic groups contribute to specific conditions is incomplete. We analyzed the genetic diversity and relationships within the Fusobacterium genus. We report recent and ancestral recombination in core genes, indicating that fusobacteria have mosaic genomes and emphasizing that taxonomic demarcation should not rely on single genes/gene regions.
View Article and Find Full Text PDFRelationship between oral microbiota and colorectal cancer: A systematic review.
J Periodontal Res
December 2024
Department of Stomatology, Faculty of Medicine and Dentistry, University of Valencia, Valencia, Spain.
This systematic review aims to investigate the microbial basis underlying the association between oral microbiota and colorectal cancer. A comprehensive search was conducted across four databases, encompassing potentially relevant studies published up to April 2024 related to the PECO question: "Is there a differentiation in oral microbial composition between adult patients diagnosed with colorectal cancer compared to healthy patients?". The Newcastle-Ottawa Scale was used to evaluate the quality of the studies included.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!