Arcelin, the antimetabolic protein from wild pulses is a known natural insecticidal molecule. Wild pulses with high arcelin content could serve as potential source to. increase the levels of insect resistance in cultivated pulse crops. In this study, arcelin (Arl) gene expression was screened in seven stored product insect pest resistant wild pulse varieties using real time RT-qPCR. Arcelin gene specific real time PCR primers were synthesized from arcelin mRNA sequence of the wild pulse variety, Lablab purpureus. The results revealed different levels of arcelin gene expression in the tested varieties. Canavalia virosa registered significantly high content indicating its suitability for utilization of arcelin gene in developing stored product insect pest resistance with other cultivated pulses.
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Pulse (Basel)
November 2024
Department of Biomedical Engineering, Cardiovascular Research Institute Maastricht (CARIM), Maastricht University, Maastricht, The Netherlands.
Introduction: Arterial stiffening is a hallmark of vascular ageing, and unravelling its underlying mechanisms has become a central theme in the field of cardiovascular disease. While various techniques and experimental setups are accessible for investigating biomechanics of blood vessels both in vivo and ex vivo, comparing findings across diverse methodologies is challenging.
Methods: Arterial stiffness in the aorta of adult (5 months) and aged (24 months) wild-type C57Bl/6J mice was measured in vivo, after which ex vivo biomechanical evaluation was performed using the Rodent Oscillatory Tension Setup to study Arterial Compliance (ROTSAC; University of Antwerp, Belgium) and the DynamX setup (Maastricht University, The Netherlands).
J Am Heart Assoc
December 2024
Department of Cardiology, Pulmonology, and Nephrology Yamagata University School of Medicine Yamagata Japan.
Background: Doxorubicin-induced cardiotoxicity is still an important medical problem associated with a high mortality rate in cancer survivors. p53 plays a key role in doxorubicin-induced cardiotoxicity. Diacylglycerol kinase ζ (Dgkζ), a 130-kDa enzyme abundant in cardiomyocytes, regulates the p53 protein expression level in neurons.
View Article and Find Full Text PDFJ Feline Med Surg
December 2024
Faculty of Veterinary Medicine of Lisbon, Lusófona University, Lisbon University Center, Portugal.
Objectives: The aim of the present study was to evaluate the differences in intraoperative nociception, incision size and operative time between midline (OVE) and flank ovariectomy (OVE) in feral or stray cats.
Methods: Two groups of animals, the OVE group (n = 19) and the OVE group (n = 19), were evaluated at six intraoperative time points. Cats assigned to both groups were premedicated with dexmedetomidine (20 μg/kg IM) and methadone (0.
Conserv Physiol
December 2024
Department of Forestry and Wildlife Management, University of Inland Norway, Evenstad, NO-2480 Koppang, Norway.
Monitoring physiological indicators including heart rate (HR) is crucial for managing animal welfare across diverse settings, from precision livestock farming to wildlife conservation. HR is a reliable indicator of energy expenditure and stress, yet the invasive nature of HR loggers limits their application in wild and free-ranging species. This study explores whether overall dynamic body acceleration (ODBA), measured with an external accelerometer, can serve as a less invasive proxy for HR.
View Article and Find Full Text PDFAuton Neurosci
December 2024
Stress Physiology Lab, Department of Chemistry, Life Sciences and Environmental Sustainability, University of Parma, Parma, Italy. Electronic address:
The identification of reliable biomarkers of transcutaneous auricular vagus nerve stimulation (taVNS) responsiveness is a key challenge both at the clinical and preclinical level. Vagally-mediated heart rate variability (vmHRV), a surrogate measure of cardiac vagal efferent activity, is an ideal candidate. Yet, the effects of taVNS on vmHRV remain inconclusive, likely due to the high degree of heterogeneity in stimulation protocols (e.
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