AI Article Synopsis
Article Abstract
Skeletal development and invasion by tumor cells depends on proteolysis of collagen by the pericellular metalloproteinase MT1-MMP. Its hemopexin-like (HPX) domain binds to collagen substrates to facilitate their digestion. Spin labeling and paramagnetic nuclear magnetic resonance (NMR) detection have revealed how the HPX domain docks to collagen I-derived triple helix. Mutations impairing triple-helical peptidase activity corroborate the interface. Saturation transfer difference NMR suggests rotational averaging around the longitudinal axis of the triple-helical peptide. Part of the interface emerges as unique and potentially targetable for selective inhibition. The triple helix crosses the junction of blades I and II at a 45° angle to the symmetry axis of the HPX domain, placing the scissile Gly∼Ile bond near the HPX domain and shifted ∼25 Å from MMP-1 complexes. This raises the question of the MT1-MMP catalytic domain folding over the triple helix during catalysis, a possibility accommodated by the flexibility between domains suggested by atomic force microscopy images.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4317567 | PMC |
| http://dx.doi.org/10.1016/j.str.2014.11.021 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Effects of polysaccharide fractions isolated from the pulp of rose laevigata Michx fruit on the foaming and polarization of RAW264.7 macrophage cells.
Int J Biol Macromol
December 2024
College of Food Science and Technology, Nanjing Agricultural University, Nanjing 210095, PR China. Electronic address:
The foaming and polarization of macrophages are pivotal in the formation and development of atherosclerosis. This study delved into the structure and membrane pattern recognition receptors (PRRs) of the neutral polysaccharide fraction (PPRLMF-1), investigating effects of PPRLMF-1 and acid polysaccharide fraction (PPRLMF-2) on the foaming and polarization of RAW264.7 macrophage cells, and exploring their underlying mechanisms.
View Article and Find Full Text PDFStudy on the Extraction Technology and Antioxidant Capacity of Polysaccharides.
Foods
December 2024
School of Public Health, Guangzhou Medical University, Xinzao, Panyu District, Guangzhou 511436, China.
A red alga named was explored, and the extraction technology and antioxidant capacity of its polysaccharides were investigated. The crude polysaccharides were extracted using the ultrasound-assisted water extraction method, precipitated by alcohol, and purified using the trichloroacetic acid method. Subsequently, the scavenging rates of polysaccharides on hydroxyl, , and free radicals, were determined both prior to and following purification to evaluate their antioxidant activity.
View Article and Find Full Text PDFTFAP2A activates CTHRC1 to influence the migration of lung adenocarcinoma cells by modulating fatty acid metabolism.
Prostaglandins Other Lipid Mediat
December 2024
Department of Thoracic Surgery, Anyang Tumor Hospital, Anyang 455000, China. Electronic address:
Background: Tumor metastasis is the main cause of death in lung adenocarcinoma (LAC) patients. It is known that the collagen triple helix repeats containing 1 (CTHRC1) protein is implicated in tissue remodeling and is tightly linked to the carcinogenesis and metastasis of solid tumors. However, the functional role of CTHRC1 and its potential mechanisms in LAC cell metastasis have not been fully explored.
View Article and Find Full Text PDFTernary complex of soluble undenatured type II collagen-hydrophobic phytochemical-chondroitin sulfate facilitates high stability and targeted intestinal release properties to active substance.
Int J Biol Macromol
December 2024
School of Food Science and Engineering, South China University of Technology, Guangzhou 510640, China; Guangdong Food Green Processing and Nutrition Regulation Technologies Research Center, Guangzhou 510650, China; Food Laboratory of Zhongyuan, Luohe 462300, Henan, China. Electronic address:
Researchers have reported that soluble undenatured type II collagen (SC II) and hydrophobic phytochemicals (HPs) can ameliorate osteoarthritis (OA) through several mechanisms. However, the solubility of HPs, the stability of SC II, and the bio-accessibility of both need to be greatly improved before they can be successfully used for this purpose. In this study, two common HPs, curcumin (CUR, a hydrophobic polyphenol) and astaxanthin (AST, a carotenoid), were first loaded into SC II, which was then complexed with chondroitin sulfate (CS) to form ternary complexes: SC II-HP-CS.
View Article and Find Full Text PDFTriple Helix Molecular Switch Cascade Multiple Signal Amplification Strategies for Ultrasensitive Chloramphenicol Detection.
Anal Chem
December 2024
Molecular Science and Biomedicine Laboratory, State Key Laboratory for Chemo/Bio-Sensing and Chemometrics, College of Material Science and Engineering, College of Chemistry and Chemical Engineering, Hunan University, Changsha, Hunan410082, China.
Article Synopsis
- A novel self-powered biosensor has been created to detect chloramphenicol (CAP) using a unique triple helix molecular switch (THMS) and DNA walkers for enhanced signal amplification.
- The biosensor employs a CAP aptamer that binds to CAP in the presence of the target, triggering a sequence of signal releases and structural changes in the DNA walker system.
- With a detection limit of 0.012 fM, this biosensor demonstrates high sensitivity for CAP detection in milk, along with excellent selectivity, stability, and reproducibility.
Want AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!