A real-time PCR for detection and quantification of M. ovipneumoniae was developed using 9 recently sequenced M. ovipneumoniae genomes and primers targeting a putative adhesin gene p113. The assay proved to be specific and sensitive (with a detection limit of 22 genomic DNA) and could quantify M. ovipneumoniae DNA over a wide linear range, from 2.2 × 10(2) to 2.2 × 10(7) genomes.
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|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4300380 | PMC |
| http://dx.doi.org/10.1292/jvms.14-0094 | DOI Listing |
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