AI Article Synopsis
- Feline morbillivirus (FmoPV) is a newly identified virus linked to chronic kidney disease in domestic cats, but its features are not well understood.
- The study developed a quantitative assay using indirect immunofluorescence to measure FmoPV viral levels, finding it stable at 4°C for up to 12 days and inactive after heat treatment.
- These findings aim to improve virus isolation methods and provide crucial insights for managing FmoPV infection risks.
Article Abstract
Feline morbillivirus (FmoPV) is an emerging virus that was recently discovered in domestic cats with chronic nephritis. Despite the potential role of FmoPV in chronic nephritis, little is known about its biological characteristics. In this study, we established a quantitative assay of FmoPV by using an indirect immunofluorescence technique. Viral titers of FmoPV were determined in one week. Treatment with polybrene® or trypsin which was previously used in virus isolation did not augment the virus titers. FmoPV was notably stable at 4°C, retaining high titers for at least 12 days. Heat-treatment at 60°C and 70°C effectively inactivated FmoPV in 10 and 2 min, respectively. The biological characteristics of FmoPV reported here will be beneficial for establishing an efficient virus isolation method and will provide important information to take a measure to reduce the risk of FmoPV infection.
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Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4478736 | PMC |
| http://dx.doi.org/10.1292/jvms.14-0623 | DOI Listing |
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