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In vitro induction of human adipose-derived stem cells into lymphatic endothelial-like cells. | LitMetric

AI Article Synopsis

  • Human adipose-derived stem cells (hADSCs) can potentially treat lymphatic edema, but there’s no standardized method for their conversion into lymphatic endothelial-like cells.
  • The study used VEGF-C156S and growth factors to induce hADSCs into lymphatic endothelial-like cells, confirming differentiation through specific cell markers and gene expression.
  • Flow cytometry analysis showed that the induced cells shared characteristics with mesenchymal stem cells, indicating successful differentiation, which could lead to future applications in lymphatic reconstruction.

Article Abstract

Human adipose-derived stem cells (hADSCs) may provide a suitable number of progenitors for the treatment of lymphatic edema; however, to date the protocols for inducing hADSCs into this tissue type have not been standardized. We wished to investigate the induction of hADSCs into lymphatic endothelial-like cells using vascular endothelial growth factor-C156S (VEGF-C156S) and other growth factors in vitro. hADSCs from healthy adult adipose tissue were purified using enzyme digestion. Differentiation was induced using medium containing VEGF-C156S and bovine fibroblast growth factor (bFGF). Differentiation was confirmed using immunostaining for lymphatic vessel endothelial hyaluronan receptor (LYVE-1) and fms-related tyrosine kinase 4 (FLT-4), two lymphatic endothelial cell markers. The expression levels of LYVE-1, prospero homeobox 1 (PROX-1), and FLT-4 throughout induction were assessed using reverse transcriptase quantitative polymerase chain reaction. hADSCs were successfully obtained by trypsin digest and purification. Flow cytometry showed these cells were similar to mesenchymal stem cells, with a high positive rate of CD13, CD29, CD44, and CD105, and a low positive rate of CD31, CD34, CD45, and HLA-DR. Induction to lymphatic endothelial-like cells was successful, with cells expressing high levels of LYVE-1, PROX-1, and FLT-4. Adipose-derived stem cells can be induced to differentiate into lymphatic endothelial-like cells using a medium containing VEGF-C156S, bFGF, and other growth factors. This population of lymphatic endothelial-like cells may be useful for lymphatic reconstruction in the future.

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Source
http://dx.doi.org/10.1089/cell.2014.0043DOI Listing

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