AI Article Synopsis

  • Traditional serotyping of Escherichia coli (E. coli) has primarily relied on slide agglutination using antisera, but newer methods like multiplex immunoassays and polymerase chain reaction (PCR) have emerged.
  • In a study analyzing 161 Shiga toxin-producing E. coli (STEC) strains from California cattle, researchers used both conventional antisera methods and two advanced Luminex technology assays.
  • The Luminex-based assays successfully serotyped 11 isolates that were previously untypeable by traditional methods, with results from the two assays mostly in agreement except for 14 isolates.

Article Abstract

Traditionally, serotyping of Escherichia coli has been performed via slide agglutination methods using antisera. More recently, multiplex immunoassays and "molecular serotyping" via polymerase chain reaction (PCR) have been validated for this purpose. In this study, the serogroups of 161 Shiga toxin-producing Escherichia coli (STEC) strains isolated from fecal samples of California cattle were typed by conventional methods using antisera as well as two newly developed multiplex PCR- and antibody-based microbead assays using the Luminex technology. Using the Luminex assays, we were capable of serotyping 11 STEC isolates that were previously determined untypeable for the O antigen by conventional methods using antisera. Except for 14 isolates, results from the 2 Luminex assays agreed.

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http://dx.doi.org/10.1089/fpd.2014.1827DOI Listing

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