Murraya exotica is a traditional Chinese medicine (TCM) widely grown in southeast China. We herein proposed a fast strategy for separation and identification of active components of cancer metastatic chemopreventives from the root, leaf, twig and stem bark extracts that were obtained by reflux in 80% acidic ethanol and then liquid-liquid extraction. High performance liquid chromatography (HPLC) analysis showed that the extract mixtures from leaf, bark and twig were similar, while the root extract contained a characteristic component (CM1). Bioactivity assays revealed that the root extract contained some active components that significantly inhibited cancer cell viability and migration. Ultra performance liquid chromatography coupled with diode array detection and electrospray ionization mass spectrometry (UPLC-DAD-ESI-MS) analysis indicated the existence of coumarins in the root and leaf extracts. Semi-preparative chromatographic separation and physicochemical characterization indicated that CM1 was a novel coumarin derivative that warrants further chemopreventive studies on cancer metastasis. The present phytochemical and phytopharmacological studies exemplify a fast strategy for screening and identifying active component(s) from raw extracts of phytomedicines.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1016/j.jpba.2015.01.023 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Molecular Mechanisms of Humic Acid in Inhibiting Silica Scaling during Membrane Distillation.
Environ Sci Technol
January 2025
Shandong Key Laboratory of Water Pollution Control and Resource Reuse, School of Environmental Science and Engineering, Shandong University, Qingdao 266237, P. R. China.
Membrane distillation (MD) efficiently desalinizes and treats high-salinity water as well as addresses the challenges in handling concentrated brines and wastewater. However, silica scaling impeded the effectiveness of MD for treating hypersaline water and wastewater. Herein, the effects of humic acid (HA) on silica scaling behavior during MD are systematically investigated.
View Article and Find Full Text PDFStructure of Hydrothermally Stable Acid Sites and their Catalytic Role in P-Modified ZSM-5 Zeolite Revealed by Solid-State NMR Spectroscopy.
Inorg Chem
January 2025
State Key Laboratory of Molecular & Process Engineering, SINOPEC Research Institute of Petroleum Processing, Beijing 100083, China.
The ZSM-5 zeolite is the key active component in high-severity fluid catalytic cracking (FCC) catalysts and is routinely activated by phosphorus compounds in industrial production. To date, however, the detailed structure and function of the introduced phosphorus still remain ambiguous, which hampers the rational design of highly efficient catalysts. In this work, using advanced solid-state NMR techniques, we have quantitatively identified a total of seven types of P-containing complexes in P-modified ZSM-5 zeolite and clearly revealed their structure, location, and catalytic role.
View Article and Find Full Text PDFIdentification of Lauric Acid as a Potent Sodium Channel Na1.5 Blocker from Compound Chinese Medicine Wenxin Keli.
Drug Des Devel Ther
January 2025
State Key Laboratory of Component-Based Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin, 301617, People's Republic of China.
Purpose: The major cardiac voltage-gated sodium channel Na1.5 (I) is essential for cardiac action potential initiation and subsequent propagation. Compound Chinese medicine Wenxin Keli (WXKL) has been shown to suppress arrhythmias and heart failure.
View Article and Find Full Text PDFThio-ProTide strategy: A novel HS donor-drug conjugate (DDC) alleviates hepatic injury innate lysosomal targeting.
Acta Pharm Sin B
December 2024
Key Laboratory of Drug Metabolism and Pharmacokinetics, Research Unit of PK-PD Based Bioactive Components and Pharmacodynamic Target Discovery of Natural Medicine of Chinese Academy of Medical Sciences, China Pharmaceutical University, Nanjing 210009, China.
Hydrogen sulfide (HS) is a gas signaling molecule with versatile bioactivities; however, its exploitation for disease treatment appears challenging. This study describes the design and characterization of a novel type of HS donor-drug conjugate (DDC) based on the thio-ProTide scaffold, an evolution of the ProTide strategy successfully used in drug discovery. The new HS DDCs achieved hepatic co-delivery of HS and an anti-fibrotic drug candidate named hydronidone, which synergistically attenuated liver injury and resulted in more sufficient intracellular drug exposure.
View Article and Find Full Text PDFTherapeutic efficacy of against phenylhydrazine-induced hemolytic anemia in rats.
J Tradit Complement Med
January 2025
School of Chemical & Biotechnology, SASTRA Deemed University, Thanjavur, India.
Background & Aim: Hemolytic anemia is a blood disorder whose incidence is increasing in the world in recent years especially after the pandemic. Conventional treatments include use of steroids and immunosuppresants that are accompanied by numerous adverse effects. With growing interest in using complex multi-component formulations for multi-targeted therapy, the present study aims to investigate the therapeutic efficacy of a traditional herbomineral preparation, , which has been traditionally used as a supplement in iron-deficiency anemia, against phenylhydrazine-induced hemolytic anemia in rodent models.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!