AI Article Synopsis
- * Experiments involved using different glucose concentrations and analyzing the impact of a protein called SDF-1 on BMSC migration, finding that high glucose reduces migration while SDF-1 generally promotes it.
- * Results indicate that high glucose not only hampers BMSC migration but also decreases the levels and expression of both CXCR-4 and MMP-2, highlighting the role of the SDF-1/CXCR-4 pathway in this process.
Article Abstract
Purpose: To explore the effect of high glucose on migration of BMSCs through inhibiting CXCR-4.
Methods: Bone marrow stromal cells (BMSCs) were obtained from the mandible of Wistar rats and stimulated with different concentrations of glucose (5.5, 16.5 mmol/L). The optimum concentration of SDF-1 was evaluated by Transwell assay in physiological glucose concentration (5.5 mmol/L). In the optimum concentration of SDF-1 condition, we detected the effect of SDF-1 and AMD3100 on migration of BMSCs in different concentrations of glucose (5.5, 16.5 mmol/L). CXCR-4 protein levels were determined by Western blot. The mRNA expression of CXCR-4 and MMP-2 were tested by RT-PCR. SPSS 11.0 software package was used for statistical analysis.
Results: The optimum concentration of SDF-1 was 100 ng/mL. High glucose could inhibit the migration of BMSCs. In different concentrations of glucose, SDF-1 could promote the migration of BMSCs, but AMD3100 could inhibit this promotion. High glucose condition could inhibit the secretion of CXCR-4 and mRNA expression of CXCR-4 and MMP-2.
Conclusions: High glucose inhibits migration of BMSCs by inhibiting CXCR-4 through SDF-1/CXCR-4 pathway.
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Article Synopsis
- The prolonged use of external fixation during distraction osteogenesis (DO) can heighten complications, while bone marrow mesenchymal stem cells (BMSCs) play a crucial role in bone regeneration due to their pro-angiogenic and osteogenic abilities.
- RSC-96, a type of Schwann cell, has been shown to promote the proliferation, migration, and differentiation of BMSCs when co-cultured, enhancing both bone formation and blood vessel development through neurotrophic factor secretion and activation of specific signaling pathways.
- In a rat DO model, RSC-96's conditioned medium improved bone healing outcomes, with notable increases in gene expression markers for osteogenesis and angiogenesis, alongside positive radiological and biomechanical assessments.
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