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Validation of plasmonic-based biosensors for rapid and in depth characterization of monoclonal antibodies directed against rabbit haemorrhagic and foot-and-mouth disease viruses in biological samples.
Methods
December 2024
Department of Molecular and Translational Medicine, University of Brescia, Brescia, Italy; Consorzio Interuniversitario Biotecnologie (CIB), Unit of Brescia, Brescia, Italy. Electronic address:
ELISA and RT-PCR represent the standard tools for the sensitive identification of viruses in biological samples, but they lack the capacity to finely characterize the binding of viruses or viral antigens to monoclonal antibodies (MAbs). Biosensing technologies are gaining increasing importance as powerful MAb characterization tools in the field of virology. Surface plasmon resonance (SPR) is an optical biosensing technology already used for the in depth characterization of MAbs of diagnostic and therapeutic value.
View Article and Find Full Text PDFFirst Detection of Benign Rabbit Caliciviruses in Chile.
Viruses
March 2024
Commonwealth Scientific and Industrial Research Organisation, Health and Biosecurity, Black Mountain, Canberra, ACT 2601, Australia.
Pathogenic lagoviruses (Rabbit hemorrhagic disease virus, RHDV) are widely spread across the world and are used in Australia and New Zealand to control populations of feral European rabbits. The spread of the non-pathogenic lagoviruses, e.g.
View Article and Find Full Text PDFSeveral conventional and recently available tools are available for an integrated control of European rabbits in Australia. We quantified the impact of the release of rabbit haemorrhagic disease virus K5 (RHDV K5, hereafter K5) and pindone (2-pivalyl-1,3-indandione) baiting at 13 sites within Cudlee Creek fire scar in the Adelaide Hills, South Australia. K5 release was followed by pindone baiting between December 2021 and March 2022; the application of both control methods followed industry best practice.
View Article and Find Full Text PDFApoptosis activation during /GI.2 infection in rabbits.
Front Microbiol
January 2024
Institute of Biology, University of Szczecin, Szczecin, Poland.
Rabbit Haemorrhagic Disease (RHD) is a severe disease caused by /GI.1 and GI.2.
View Article and Find Full Text PDFUtilizing Molecular Epidemiology and Citizen Science for the Surveillance of Lagoviruses in Australia.
Viruses
November 2023
Commonwealth Scientific and Industrial Research Organisation, Health and Biosecurity, Canberra, ACT 2601, Australia.
Australia has multiple lagoviruses with differing pathogenicity. The circulation of these viruses was traditionally determined through opportunistic sampling events. In the lead up to the nationwide release of RHDVa-K5 (GI.
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