Purpose: To measure membrane attraction capabilities of enhanced 27-gauge, enhanced 25-gauge, and 23-gauge vitrectomy probes under various parameters.
Methods: A membrane-on-cantilever apparatus was used to measure membrane attraction for enhanced 27-, enhanced 25-, and 23-gauge UltraVit probes (n = 6 for each). The following parameters were evaluated: effects of cut rates and duty cycles on membrane attraction distances, and flow rates and vacuum levels required to attract a membrane at a fixed distance.
Results: The enhanced 27-gauge probe had the shortest attraction distance across all cutting speeds and duty cycles. To attract a membrane at a fixed distance, increasing vacuum was necessary with higher cutting rates and smaller probe gauges but flow rate remained relatively constant. The biased open duty cycle was associated with a longer attraction distance than 50/50 or biased closed modes.
Conclusion: The shorter membrane attraction distance of the enhanced 27-gauge probe versus 23-gauge and enhanced 25-gauge probes may permit greater membrane dissection precision while providing improved access to small tissue planes. Equivalent fluid flow capabilities of the 27-gauge probe compared with the 23-gauge and 25-gauge probes may provide efficient aspiration. Surgeon selection of duty cycle modes may improve intraoperative fluid control and expand the cutter utility as a multifunctional tool.
Download full-text PDF |
Source |
---|---|
http://dx.doi.org/10.1097/IAE.0000000000000411 | DOI Listing |
Background: Convergent evidence indicates that deficits in the endosomal recycling pathway underlies pathogenesis of Alzheimer's disease (AD). SORL1 encodes the retromer-associated receptor SORLA that plays an essential role in recycling of AD-associated cargos such as the amyloid precursor protein and the glutamatergic AMPA receptor. Importantly, loss of function pathogenic SORL1 variants are associated with AD.
View Article and Find Full Text PDFFront Immunol
January 2025
Molecular Immunology and Gene Therapy, Max Delbrück Center for Molecular Medicine in the Helmholtz Association (MDC), Berlin, Germany.
Generation of high avidity T cell receptors (TCRs) reactive to tumor-associated antigens (TAA) is impaired by tolerance mechanisms, which is an obstacle to effective T cell therapies for cancer treatment. NY-ESO-1, a human cancer-testis antigen, represents an attractive target for such therapies due to its broad expression in different cancer types and the restricted expression in normal tissues. Utilizing transgenic mice with a diverse human TCR repertoire, we isolated effective TCRs against NY-ESO-1 restricted to HLA-A*02:01.
View Article and Find Full Text PDFACS Appl Mater Interfaces
January 2025
Department of Chemistry, University of Victoria, 3800 Finnerty Road, Victoria, BC V8P 5C2, Canada.
One of the key technical challenges before the widespread adoption of proton exchange membrane fuel cells (PEMFCs) is increasing the durability of the platinum catalyst layer to meet a target of 8000 operating hours with only a 10% loss of performance. Carbon corrosion, one of the primary mechanisms of degradation in fuel cells, has attracted attention from researchers interested in solving the durability problem. As such, the development of catalyst supports to avoid this issue has been a focus in recent years, with interest in hydrophobic supports such as highly graphitized carbons.
View Article and Find Full Text PDFMicroorganisms
November 2024
Laboratorio di Patologia Vegetale Molecolare, Dipartimento di Scienze e Tecnologie Agrarie, Alimentari Ambientali e Forestali, Università degli Studi di Firenze, Via della Lastruccia 10, 50019 Sesto Fiorentino, Firenze, Italy.
In recent years, membrane transporters have attracted considerable interest regarding their involvement in the molecular dialogue occurring between microbes and their hosts. In particular, the multidrug and toxic compound extrusion (MATE) transporters form a family of integral membrane proteins, mainly involved in the efflux of toxic and xenobiotic compounds. They are present in all living organisms, both prokaryotes and eukaryotes, where they have a wide array of extremely different roles.
View Article and Find Full Text PDFLife (Basel)
December 2024
Division of Microbiology and Molecular Biology, Medical Faculty, Private Sigmund Freud University, Freudplatz 3, 1020 Wien, Austria.
The attractiveness of OMVs derived from Gram-negative bacteria lies in the fact that they have two biomembranes sandwiching a peptidoglycan layer. It is well known that the envelope of OMVs consists of the outer bacterial membrane [OM] and not of the inner one [IM] of the source bacterium. This implies that all outer membranous molecules found in the OM act as antigens.
View Article and Find Full Text PDFEnter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!