Recombinant protein overexpression of large proteins in bacteria often results in insoluble and misfolded proteins directed to inclusion bodies. We report the application of shear stress in micrometer-wide, thin fluid films to refold boiled hen egg white lysozyme, recombinant hen egg white lysozyme, and recombinant caveolin-1. Furthermore, the approach allowed refolding of a much larger protein, cAMP-dependent protein kinase A (PKA). The reported methods require only minutes, which is more than 100 times faster than conventional overnight dialysis. This rapid refolding technique could significantly shorten times, lower costs, and reduce waste streams associated with protein expression for a wide range of industrial and research applications.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4388321 | PMC |
| http://dx.doi.org/10.1002/cbic.201402427 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Detection of Tilapia parvovirus in farm-reared tilapia in India and its isolation using fish cell lines.
In Vitro Cell Dev Biol Anim
January 2025
Aquatic Animal Health Laboratory, PG & Research Department of Zoology, C. Abdul Hakeem College, (Affiliated to Thiruvalluvar University), Melvisharam, Tamil Nadu, India.
Tilapia parvovirus (TiPV) is an emerging viral pathogen and responsible for severe economic loss in tilapia culture production. Lethargic, cutaneous haemorrhages; ocular lesions; discolouration of gill and cloudy eye and exophthalmia are common symptoms of TiPV. The TiPV-suspected tilapia fish were collected from grow-out ponds situated in different parts of Tamil Nadu, India, and screened for TiPV by PCR.
View Article and Find Full Text PDFEthics of Procuring and Using Organs or Tissue from Infants and Newborns for Transplantation, Research, or Commercial Purposes: Protocol for a Bioethics Scoping Review.
Wellcome Open Res
December 2024
National University of Singapore, Singapore, Singapore.
Unlabelled: Since the inception of transplantation, it has been crucial to ensure that organ or tissue donations are made with valid informed consent to avoid concerns about coercion or exploitation. This issue is particularly challenging when it comes to infants and younger children, insofar as they are unable to provide consent. Despite their vulnerability, infants' organs and tissues are considered valuable for biomedical purposes due to their size and unique properties.
View Article and Find Full Text PDFDistinct subcellular localization of tau and alpha-synuclein in lewy body disease.
Acta Neuropathol Commun
January 2025
Center for Neurodegeneration and Experimental Therapeutics, Department of Neurology, University of Alabama at Birmingham, Birmingham, AL, USA.
Lewy bodies and neurofibrillary tangles, composed of α-synuclein (α-syn) and tau, respectively, often are found together in the same brain and correlate with worsening cognition. Human postmortem studies show colocalization of α-syn and tau occurs in Lewy bodies, but with limited effort to quantify colocalization. In this study, postmortem middle temporal gyrus tissue from decedents (n = 9) without temporal lobe disease (control) or with Lewy body disease (LBD) was immunofluorescently labeled with antibodies to phosphorylated α-syn (p-α-syn), tau phosphorylated at Ser202/Thr205 (p-tau), or exposure of tau's phosphatase-activating domain (PAD-tau) as a marker of early tau aggregates.
View Article and Find Full Text PDFEmergence of West Nile virus infection in Kerala, South India: A case of concern.
J Vector Borne Dis
January 2025
ICMR-Vector Control Research Centre Field Station, Kottayam, Kerala, India.
West Nile virus (WNV) infection is emerging as a disease of public health concern in Kerala, India with recurring outbreaks since 2011. With its tropical climate, biodiversity hot spots of Western ghats, forest cover, plenty of water bodies and bird sanctuaries, Kerala provides an ideal ecological niche for vector breeding and transmission of WNV. In this article, we reflect on the peculiar features of the outbreaks of WNV fever in Kerala and highlight the knowledge gaps, the research priorities and the need for effective control measures.
View Article and Find Full Text PDFFowl adenovirus 8a isolated from chickens with runting and stunting syndrome induces inclusion body hepatitis and hepatitis-hydropericardium syndrome in chicken embryos.
Vet World
November 2024
Laboratory of Avian Diseases, School of Veterinary Medicine and Animal Science, Department of Pathology, University of São Paulo, São Paulo, Brazil.
Background And Aim: Fowl adenovirus (FAdV) is the etiological agent of inclusion body hepatitis (IBH) and hepatitis-hydropericardium syndrome (HHS) in poultry. It is also detected in chickens with runting and stunting syndrome (RSS). FAdV has been detected worldwide, and genotypes 8a, 8b, and 11 have been identified in chickens with enteric problems in Brazil.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!