AI Article Synopsis
- * Researchers developed a sensitive high-performance liquid chromatography method with fluorescence detection to measure acrolein levels in human serum through pre-column derivatization using a specific reagent.
- * This method allows for clear detection of acrolein without interference from other substances, achieving a detection limit of 10 nM while requiring only a straightforward protein precipitation step.
Article Abstract
Acrolein is a major unsaturated aldehyde that is generated during the lipid peroxidation process. The measurement of acrolein in biological samples should be useful to estimate the degree of lipid peroxidation and to evaluate the effect of hazardous properties of acrolein on human health. In this study, a highly sensitive and selective high-performance liquid chromatography with fluorescence detection method was developed for the determination of acrolein in human serum. The proposed method involves the pre-column fluorogenic derivatization of acrolein with 1,2-diamino-4,5-dimethoxybenzene (DDB) as a reagent. The fluorescent derivative of acrolein could be detected clearly without any interfering reagent blank peaks because DDB does not have intrinsic fluorescence itself, and the detection limit was 10 nM (signal-to-noise ratio = 3). The proposed method could selectively detect acrolein in human serum with a simple protein precipitation treatment.
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| http://dx.doi.org/10.1002/bmc.3422 | DOI Listing |
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