AI Article Synopsis
- The study focused on using fluorescence sequencing typing technology to identify the ingredients in Liuwei Dihuang pill, a type of Chinese patent medicine.
- The researchers amplified and sequenced DNA from the plant Paeonia suffruticosa using specially designed primers, analyzing the results with GeneMarker software.
- The psbA-trnH fluorescence labeling primer proved effective, successfully identifying three key medicinal plants in the Liuwei Dihuang pill, highlighting the reliability of this method for distinguishing raw materials in traditional Chinese medicine.
Article Abstract
In this paper, Liuwei Dihuang pill was used to study the identification of Chinese patent medicine by fluorescence sequencing typing technology. The DNA of Paeonia suffruticosa was used as template to amplify by five pair of FAM fluorescence labeling primers. Then, the amplified products were sequenced. The sequencing results were analyzed by GeneMarker V1.80 to screen the best fluorescence labeling primers. As a result, psbA-trnH fluorescence labeling primer was used to identify the raw materials of Liuwei Dihuang pill. The results showed that three kinds of raw plant medicinal materials in Liuwei Dihuang pill were able to be correctly identified by psbA-trnH fluorescence labeling primer. The fluorescence sequencing typing technology can stably and accurately distinguish raw medicinal materials in Chinese patent medicine.
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