AI Article Synopsis
- Researchers developed a PCR method to distinguish between Cistanche deserticola, Cynomorium songaricum, and Orobanche pycnostachya using specific alleles.
- A total of 30 C. deserticola samples, 21 C. songaricum samples, and samples of O. pycnostachya were collected, and DNA was extracted for amplification and sequencing.
- The optimized PCR reaction showed distinct identification for C. deserticola with a 331 bp band, demonstrating the method's effectiveness compared to traditional identification techniques.
Article Abstract
To explore the new method of discriminating Cistanche deserticola, Cynomorium songaricum and Orobanche pycnostachya by using PCR amplification of specific alleles. 30 samples of the different C. deserticola, 21 samples of C. songaricum and O. pycnostachya were collected. The total DNA of the samples were extracted, the ITS sequences from C. deserticola, C. songaricum and O. pycnostachya were amplified by PCR and sequenced unidirectionally. These sequences were aligned by using ClustulW. Specific primer was designed according to the ITS sequences of specific alleles, and PCR reaction system was optimized. Additionally, compare with the identification of specific PCR method and DNA sequence analysis method. The result showed that the 331 bp identification band for C. deserticola and the adulterants not amplified bands by a single PCR reaction, which showed good identification ability to the three species. PCR amplification of specific alleles can be used to identify C. deserticola, C. songaricum and O. pycnostachya successfully.
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