The discrimination of Leishmania species from patient samples has epidemiological and clinical relevance. In this study, different gene target PCR-restriction fragment length polymorphism (RFLP) protocols were evaluated for their robustness as Leishmania species discriminators in 61 patients with cutaneous leishmaniasis. We modified the hsp70-PCR-RFLP protocol and found it to be the most reliable protocol for species identification.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4365241 | PMC |
| http://dx.doi.org/10.1128/JCM.02955-14 | DOI Listing |
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