Various analytes can be verified by surface plasmon resonance, thus continuous improvement of this sensing technology is crucial for better sensing selection and higher sensitivity. The SPR sensitivity on the wavelength modulation is enhanced with increasing wavelengths. The telecommunication wavelength range was then utilized to detect Mycobacterium tuberculosis (MTB) deoxyribonucleic acid (DNA) under two situations, without immobilization and with 5'-thiol end labeled IS6100 DNA probes, for SPR sensitivity comparison. The experimental data demonstrated that the SPR sensitivity increased more than 13 times with the wavelength modulation after immobilization. Since the operating wavelength accuracy of a tunable laser source can be controlled within 0.001 nm, the sensitivity and resolution on immobilized MTB DNA were determined as 1.04 nm/(μg/mL) and 0.9 ng/mL, respectively.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4327021 | PMC |
| http://dx.doi.org/10.3390/s150100331 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
A SPR aptamer sensor for mercury based on AuNPs@NaYF:Yb,Tm,Gd upconversion luminescent nanoparticles.
Anal Methods
November 2017
Anhui Key Laboratory of Chemo-Biosensing, Key Laboratory of Functional Molecular Solids, Ministry of Education, College of Chemistry and Materials Science, Anhui Normal University, Wuhu 241000, PR China.
A new aptamer-based surface plasmon resonance (SPR) system has been designed to detect Hg that utilizes near-infrared (NIR)-to-NIR gold nanoparticle coated NaYF:Yb,Tm,Gd up-conversion nanoparticles (AuNPs@NaYF:Yb,Tm,Gd UCNPs) as probes. The AuNPs@NaYF:Yb,Tm,Gd UCNPs were prepared and excited by near-infrared light (980 nm) which emitted at a near-infrared wavelength (808 nm) using an inexpensive infrared continuous wave laser diode. The AuNPs@NaYF:Yb,Tm,Gd UCNPs were conjugated with Hg aptamers.
View Article and Find Full Text PDFChimeric antigen receptor macrophages (CAR-M) sensitize HER2+ solid tumors to PD1 blockade in pre-clinical models.
Nat Commun
January 2025
Carisma Therapeutics Inc, Philadelphia, PA, USA.
We previously developed human CAR macrophages (CAR-M) and demonstrated redirection of macrophage anti-tumor function leading to tumor control in immunodeficient xenograft models. Here, we develop clinically relevant fully immunocompetent syngeneic models to evaluate the potential for CAR-M to remodel the tumor microenvironment (TME), induce T cell anti-tumor immunity, and sensitize solid tumors to PD1/PDL1 checkpoint inhibition. In vivo, anti-HER2 CAR-M significantly reduce tumor burden, prolong survival, remodel the TME, increase intratumoral T cell and natural killer (NK) cell infiltration, and induce antigen spreading.
View Article and Find Full Text PDFStructure switching aptamer enhance sensitivity and specificity of photonic crystal-based sensors for RSV-G protein detection.
Biosens Bioelectron
January 2025
Beijing Institute of Technology School of Chemistry and Chemical Engineering, China. Electronic address:
Photonic crystal-based aptasensors for viral proteins detection offer the advantage of producing visible readouts. However, they usually suffer from limited sensitivity and high non-specific background noise. A significant contributing factor to these issues is the use of fixed-conformation aptamers in these sensors.
View Article and Find Full Text PDFA Method for Imaging the Ischemic Penumbra with MRI using IVIM.
AJNR Am J Neuroradiol
January 2025
From the Department of Radiology, Medical Physics (MML, TJC), Department of Interventional Radiology (NS, GAC), Department of Surgery and Large Animal Studies (MAN), and the Department of Statistics (MG), University of Chicago, Chicago, IL, USA; Department of Anesthesiology (SPR), University of Illinois, Chicago, IL, USA; Department of Radiology (MSS), University of Massachusetts Chan Medical School, Worcester, MA, USA; Department of Radiology, Biomedical Engineering and Imaging Institute (Current affiliation MML), Icahn School of Medicine at Mount Sinai, New York, NY, USA; Mount Carmel Health Systems (Current affiliation GAC), Columbus, OH, USA.
Background And Purpose: In acute ischemic stroke, the amount of "local" CBF distal to the occlusion, i.e. all blood flow within a region whether supplied antegrade or delayed and dispersed through the collateral network, may contain valuable information regarding infarct growth rate and treatment response.
View Article and Find Full Text PDFChromium Detection in Water Using Optical Methods: A Study of Reagent and Reagentless Approaches.
Crit Rev Anal Chem
January 2025
Department of Electronics and Communication, Vidyavardhaka College of Engineering, Mysuru, India.
Water contaminated with chromium (Cr) poses significant risks to public health and the environment, necessitating reliable detection techniques. This review study uniquely provides a comprehensive analysis of optical methods for detecting Cr pollution in water, focusing on both reagent-based and reagentless approaches, as well as various sensing platforms. Unlike existing reviews that primarily focus on electrochemical and colorimetric/fluorimetric methods, this work highlights the untapped potential of optical technologies, such as colorimetry, SPR, UV-Vis spectroscopy, and more, in detecting distinct Cr species, including reagent and reagentless based approaches.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!