Premise Of The Study: Microsatellite primers were developed for the parasitic Psittacanthus schiedeanus, a common mistletoe species on cloud forest-adapted tree hosts in Mesoamerica, to investigate intraspecific genetic patterns of diversity and genetic structure. •
Methods And Results: Using an enriched library, 10 polymorphic microsatellite loci were developed in P. schiedeanus. All loci consisted of dinucleotide repeats. Average alleles per locus were 12 (4-17), and a total of 120 alleles were recorded across 39 individuals from four populations in Mexico. Primers were tested in 11 additional species, but only amplified successfully in P. calyculatus and P. angustifolius. •
Conclusions: The polymorphic loci described will be useful in studies of genetic diversity and genetic population differentiation in natural populations of these parasitic plants, and will provide valuable information to understand the importance of host distribution.
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http://dx.doi.org/10.3732/apps.1400099 | DOI Listing |
PLoS Genet
December 2024
Laboratory of Genetics, University of Wisconsin-Madison, Madison, Wisconsin, United States of America.
Advances in DNA sequencing technology and computation now enable genome-wide scans for natural selection to be conducted on unprecedented scales. By examining patterns of sequence variation among individuals, biologists are identifying genes and variants that affect fitness. Despite this progress, most population genetic methods for characterizing selection assume that variants mutate in a simple manner and at a low rate.
View Article and Find Full Text PDFPlants (Basel)
December 2024
Centre for the Research and Technology of Agro-Environmental and Biological Sciences (CITAB), University of Trás-os-Montes e Alto Douro (UTAD), 5000 Vila Real, Portugal.
The genus contains about 500 species distributed worldwide but only a limited number of species have been studied for genetic diversity using molecular markers. In this study, a genetic analysis was conducted on three Vaccinium species (four cultivars of , four wild populations of , and two cultivars of ), for a total of 95 genotypes, using eight microsatellite (SSR) loci. A total of 57 alleles were detected.
View Article and Find Full Text PDFInt J Mol Sci
December 2024
National Key Laboratory for Tropical Crop Breeding, Key Laboratory of Tropical Fruit Biology, Ministry of Agriculture and Rural Affairs, Key Laboratory of Hainan Province for Postharvest Physiology and Technology of Tropical Horticulture Products, South Subtropical Crops Research Institute, Chinese Academy of Tropical Agricultural Sciences, Zhanjiang 524013, China.
Mango ( L.) (2n = 40) is an important perennial fruit tree in tropical and subtropical regions. The lack of information on genetic diversity at the molecular level hinders efforts in mango genetic improvement and molecular marker-assisted breeding.
View Article and Find Full Text PDFGenes (Basel)
December 2024
China National Rodent Laboratory Animal Resources Center, Beijing 102629, China.
The genetic quality of laboratory mice may have a direct impact on the results of research. Therefore, it is essential to improve genetic monitoring methods to guarantee research quality. However, few current methods boast high efficiency, high throughput, low cost, and general applicability at the same time.
View Article and Find Full Text PDFGenes (Basel)
December 2024
Laboratoire Biodiversité et Génomique Fonctionnelle, Faculté des Sciences, Université Saint-Joseph, Campus Sciences et Technologies, Mar Roukos, Mkalles, P.O. Box 1514, Riad el Solh, Beirut 1107 2050, Lebanon.
Background/objectives: Lebanon, one of the oldest centers of grapevine ( L.) cultivation, is home to a rich diversity of local grape varieties. This biodiversity is linked to the country's unique topography and millennia of cultural history.
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