AI Article Synopsis
- MicroRNAs, like miR-155, are small RNA molecules that regulate gene expression and play key roles in processes such as cell proliferation and apoptosis, impacting immune response and inflammation.
- This study focused on the effects of inhibiting miR-155 in Jurkat cells, using various concentrations of an inhibitor to assess cell sensitivity, measure caspase-3 activity, and analyze miR-155 expression through QRT-PCR.
- Results showed that higher concentrations of the miR-155 inhibitor significantly increased caspase-3 activity, suggesting that targeting miR-155 could induce apoptosis and potentially serve as a therapeutic approach in cancer treatment.
Article Abstract
Background: MicroRNAs are small and non-coding RNA molecules with approximately 22 nt in length that cause inhibition of translation or degradation of mRNA. MiR-155 is a kind of molecule with different functions, such as its role in proliferation, apoptosis, inflammation, differentiation, and immunity. One of its best known functions is apoptosis that affects on caspase-3 activity. The main aim of this study was evaluation of miR-155 inhibition effect on cell proliferation and apoptosis induction in Jurkat cells.
Material And Methods: In this study, Jurkat cells along with MTT assay were used for evaluation of sensitivity to varied concentrations of miR-155 inhibitor (25, 50 and 75 nmol). MiR-155 expression level was analyzed using the quantitative real-time polymerase chain reaction (QRT-PCR). Caspase-3 activity was measured by caspase-3 colorimetric activity assay kit. Unpaired t-test was applied for the analysis of MTT and apoptosis results. Probability of 5% was assumed as statistically significant.
Results: According to our results, the use of miR-155 inhibitor increased the activity of caspase-3 by 2 fold in 75 nmol concentration. In this research, we found that the proper increase of miR-155 inhibitor concentration can inhibit miR-155 and consequently increase caspase-3 activity and induce apoptosis in the Jurkat cells leading to cell death ultimately.
Conclusions: Apoptosis induction by miRNAs activation or inhibition is probably one of the best and low risk ways of cell death induction in malignancies. Due to role of miR-155 in several cancer cells, it may be used as a therapeutic target in future.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4293513 | PMC |
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